Development of a prototype wound dressing technology which can detect and report colonization by pathogenic bacteria

被引：44

作者：

Zhou, Jin ^{[1
]}

Thet Naing Tun ^{[1
]}

Hong, Sung-ha ^{[1
]}

Mercer-Chalmers, June D. ^{[1
]}

Laabei, Maisem ^{[1
]}

Young, Amber E. R. ^{[2
]}

Jenkins, A. Tobias A. ^{[1
]}

机构：

[1] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

[2] N Bristol NHS Trust, SW Childrens Burns Ctr, Frenchay Hosp, Bristol BS16 1LE, Avon, England

来源：

BIOSENSORS & BIOELECTRONICS | 2011年 / 30卷 / 01期

关键词：

Wound-dressing; Infection; Staphylococcus aureus; Pseudomonas aeruginosa; Phospholipid vesicle; TOXIC-SHOCK-SYNDROME; PREVENTION; DIAGNOSIS; DELIVERY;

D O I：

10.1016/j.bios.2011.08.028

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A new methodology for detecting the microbiological state of a wound dressing in terms of its colonization with pathogenic bacteria such as Staphylococcus aureus or Pseudomonas aeruginosa has been developed. Here we report how stabilized lipid vesicles containing self-quenched carboxyfluorescein dye are sensitive to lysis only by toxins/virulence factors from P. aeruginosa and S. aureus but not by a non-toxic Escherichia coli species. The development of the stabilized vesicles is discussed and their response to detergent (triton), bacterial toxin (alpha-hemolysin) and lipases (phospholipase A(2)). Finally, fabrics with stabilized vesicles attached via plasma deposited maleic anhydride coupling are shown visibly responding to S. aureus (MSSA 476) and P. aeruginosa (PAO1) but not E. coli DH5 alpha in a prototype dressing. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：67 / 72

页数：6

共 24 条

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