Endotoxin stimulates monocyte-endothelial cell interactions in mouse intestinal Peyer's patches and villus mucosa

被引：9

作者：

Ishii, N ^{[1
]}

Tsuzuki, Y ^{[1
]}

Matsuzaki, K ^{[1
]}

Miyazaki, J ^{[1
]}

Okada, Y ^{[1
]}

Hokari, R ^{[1
]}

Kawaguchi, A ^{[1
]}

Nagao, S ^{[1
]}

Itoh, K ^{[1
]}

Miura, S ^{[1
]}

机构：

[1] Natl Def Med Coll, Dept Internal Med 2, Saitama, Japan

来源：

CLINICAL AND EXPERIMENTAL IMMUNOLOGY | 2004年 / 135卷 / 02期

关键词：

ICAM-1; LPS; monocyte; P-selectin; VCAM-1;

D O I：

10.1111/j.1365-2249.2003.02369.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Although monocyte-endothelial cell interactions represent an initial step in controlling the recruitment of monocytes in inflamed tissues, their dynamic processes in microvessels of lymphoid (Peyer's patches) and non-lymphoid (villus) regions in gut-associated lymphoid tissue remain poorly understood. We monitored the migration of fluorescence-labelled monocytes derived from the spleen in intestinal microvessels with or without lipopolysaccharide (LPS) treatment and investigated the role of adhesion molecules, P-selectin, vascular cell adhesion molecule (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). In control mice, there were few interactions between infused monocytes and the endothelium of intestinal microvessels. The monocyte-endothelial interactions (both rolling and adhesion) were significantly increased in intestinal microvessels of LPS-treated mice compared with those in controls. Anti-P-selectin monoclonal antibody (MoAb) significantly suppressed the LPS-induced increase in monocyte rolling in postcapillary venules of Peyer's patches and submucosal venules. Anti-VCAM-1 MoAbs significantly suppressed the LPS-induced increase in monocyte adhesion to postcapillary venules (PCVs) of Peyer's patches, submucosal venules, and villus capillaries. In contrast, anti-ICAM-1 MoAb significantly suppressed the number of adherent monocytes in PCV of Peyer's patches but not in submucosal venules or villus capillaries. These observations demonstrated that LPS treatment resulted in a significant increase in recruitment of monocytes both in microvessels of lymphoid and non-lymphoid regions and that P-selectin, VCAM-1 and ICAM-1 appeared to play important roles in LPS-induced interactions.

引用

页码：226 / 232

页数：7

共 37 条

[1] F4-80, A MONOCLONAL-ANTIBODY DIRECTED SPECIFICALLY AGAINST THE MOUSE MACROPHAGE [J].