Pi sampling: a methodical and flexible approach to initial macromolecular crystallization screening

被引：18

作者：

Gorrec, Fabrice ^{[1
]}

Palmer, Colin M. ^{[1
]}

Lebon, Guillaume ^{[1
]}

Warne, Tony ^{[1
]}

机构：

[1] MRC Lab Mol Biol, Cambridge CB2 0QH, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2011年 / 67卷

关键词：

PROTEIN-COUPLED RECEPTOR; MEMBRANE-PROTEINS; BIOLOGY;

D O I：

10.1107/S0907444911008754

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Pi sampling method is derived from the incomplete factorial approach to macromolecular crystallization screen design. The resulting 'Pi screens' have a modular distribution of a given set of up to 36 stock solutions. Maximally diverse conditions can be produced by taking into account the properties of the chemicals used in the formulation and the concentrations of the corresponding solutions. The Pi sampling method has been implemented in a web-based application that generates screen formulations and recipes. It is particularly adapted to screens consisting of 96 different conditions. The flexibility and efficiency of Pi sampling is demonstrated by the crystallization of soluble proteins and of an integral membrane-protein sample.

引用

页码：463 / 470

页数：8

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