16S rRNA-Targeted identification of cyanobacterial genera using oligonucleotide-probes immobilized on bacterial magnetic particles

被引：12

作者：

Matsunaga, T ^{[1
]}

Takeyama, H ^{[1
]}

Nakayama, H ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan

来源：

JOURNAL OF APPLIED PHYCOLOGY | 2001年 / 13卷 / 04期

关键词：

16S rRNA; bacterial magnetic particles (BMPs); cyanobacteria; identification; magnetic-capture hybridization;

D O I：

10.1023/A:1017990518648

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

16S rRNA-targeted identification of cyanobacterial genera, Anabaena, Microcystis, Nostoc, Oscillatoria, Synechococcus was developed using bacterial magnetic particles (BMPs). 16S rRNA-targeted capture probes designed from the genus specific region of the 16S rRNA sequence were immobilized on BMPs. Identification of cyanobacteria was per-formed by a sandwich hybridization using the capture probes-BMP conjugates and a digoxigenin (DIG)-labeled detector probe complementary to the highly conserved 16S rRNA sequence for cyanobacteria. The luminescence intensity of the probe/target-BMP hybrids was measured after reaction with alkaline phosphatase conjugated anti-DIG antibody. Five species of cyanobacteria from five different genera were successfully discriminated using this magnetic capture system.

引用

页码：389 / 394

页数：6

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