16S rRNA-Targeted identification of cyanobacterial genera using oligonucleotide-probes immobilized on bacterial magnetic particles

被引：12
|
作者：
Matsunaga, T [1 ]
Takeyama, H [1 ]
Nakayama, H [1 ]
机构：
[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan
来源：
JOURNAL OF APPLIED PHYCOLOGY | 2001年 / 13卷 / 04期
关键词：
16S rRNA; bacterial magnetic particles (BMPs); cyanobacteria; identification; magnetic-capture hybridization;
D O I：
10.1023/A:1017990518648
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
16S rRNA-targeted identification of cyanobacterial genera, Anabaena, Microcystis, Nostoc, Oscillatoria, Synechococcus was developed using bacterial magnetic particles (BMPs). 16S rRNA-targeted capture probes designed from the genus specific region of the 16S rRNA sequence were immobilized on BMPs. Identification of cyanobacteria was per-formed by a sandwich hybridization using the capture probes-BMP conjugates and a digoxigenin (DIG)-labeled detector probe complementary to the highly conserved 16S rRNA sequence for cyanobacteria. The luminescence intensity of the probe/target-BMP hybrids was measured after reaction with alkaline phosphatase conjugated anti-DIG antibody. Five species of cyanobacteria from five different genera were successfully discriminated using this magnetic capture system.
引用
收藏
页码：389 / 394
页数：6
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