Phylogenetic analysis of the hypervariable region of the 18S rRNA gene of Cryptosporidium oocysts in feces of Canada geese (Branta canadensis):: Evidence for five novel genotypes

被引:61
作者
Jellison, KL
Distel, DL
Hemond, HF
Schauer, DB
机构
[1] MIT, Dept Civil & Environm Engn, Cambridge, MA 02139 USA
[2] MIT, Biol Engn Div, Cambridge, MA 02139 USA
[3] MIT, Div Comparat Med, Cambridge, MA 02139 USA
[4] Univ Maine, Dept Biochem Microbiol & Mol Biol, Orono, ME 04469 USA
关键词
D O I
10.1128/AEM.70.1.452-458.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To assess genetic diversity in Cryptosporidium oocysts from Canada geese, 161 fecal samples from Canada geese in the United States were analyzed. Eleven (6.8%) were positive for Cryptosporidium spp. following nested PCR amplification of the hypervariable region of the 18S rRNA gene. Nine PCR products from geese were cloned and sequenced, and all nine diverged from previously reported Cryptosporidium 18S rRNA gene sequences. Five sequences were very similar or identical to each other but genetically distinct from that of Cryptosporidium baileyi; two were most closely related to, but genetically distinct from, the first five; and two were distinct from any other sequence analyzed. One additional sequence in the hypervariable region of the 18S rRNA gene isolated from a cormorant was identical to that of C. baileyi. Phylogenetic analysis provided evidence for new genotypes of Cryptospotidium species in Canada geese. Results of this study suggest that the taxonomy of Cryptosporidium species in geese is complex and that a more complete understanding of genetic diversity among these parasites will facilitate our understanding of oocyst sources and species in the environment.
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页码:452 / 458
页数:7
相关论文
共 32 条
[1]  
Alderisio KA, 1999, APPL ENVIRON MICROB, V65, P5628
[2]   DIFFERENTIATION BETWEEN HUMAN AND ANIMAL STRAINS OF CRYPTOSPORIDIUM-PARVUM USING ISOENZYME TYPING [J].
AWADELKARIEM, FM ;
ROBINSON, HA ;
DYSON, DA ;
EVANS, D ;
WRIGHT, S ;
FOX, MT ;
MCDONALD, V .
PARASITOLOGY, 1995, 110 :129-132
[3]   GenBank [J].
Benson, DA ;
Boguski, MS ;
Lipman, DJ ;
Ostell, J ;
Ouellette, BFF .
NUCLEIC ACIDS RESEARCH, 1998, 26 (01) :1-7
[4]   Identification of genetic heterogeneity in the Cryptosporidium parvum ribosomal repeat [J].
Carraway, M ;
Tzipori, S ;
Widmer, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (02) :712-716
[5]   Cryptosporidium in farmed animals:: the detection of a novel isolate in sheep [J].
Chalmers, RM ;
Elwin, K ;
Reilly, WJ ;
Irvine, H ;
Thomas, AL ;
Hunter, PR .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2002, 32 (01) :21-26
[6]  
Dieter R A Jr, 2001, Int J Circumpolar Health, V60, P676
[7]  
Dubey JP, 1990, CRYPTOSPORIDIOSIS MA
[8]  
Egyed Z, 2002, J PARASITOL, V88, P237, DOI 10.1645/0022-3395(2002)088[0237:PTOCBA]2.0.CO
[9]  
2
[10]  
Graczyk TK, 1998, APPL ENVIRON MICROB, V64, P2736