Synthesis and study of the fluorescein conjugate of the nucleotide dPTP

被引：1

作者：

Cummins, WJ ^{[1
]}

Hamilton, AL ^{[1
]}

Smith, CL ^{[1
]}

Briggs, MSJ ^{[1
]}

机构：

[1] Amersham Pharmacia Biotech UK Ltd, Little Chalfont HP7 9NA, Bucks, England

来源：

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期

关键词：

D O I：

10.1081/NCN-100002489

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The synthesis of a fluorescein conjugate on the non-natural base P is described. The ability of the newly synthesised fluorescein-dPTP and of a fluorescein-11-dUTP to compete with the natural nucleotide TTP was also studied. Overall the efficiency of labelling a nucleic acid with a fluorescein moiety was found to be approximately equal.

引用

页码：1049 / 1051

页数：3