Allitridi induces apoptosis by affecting Bcl-2 expression and caspase-3 activity in human gastric cancer cells

被引：0

作者：

Lan, H ^{[1
]}

Lü, YY ^{[1
]}

机构：

[1] Peking Univ, Beijing Inst Canc Res, Sch Oncol, Beijing 100034, Peoples R China

来源：

ACTA PHARMACOLOGICA SINICA | 2004年 / 25卷 / 02期

关键词：

human gastric cancer cell line; apoptosis; diallyl trisulfide; Bcl-2; CPP32; protein;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AIM: To investigate the mechanism of allitridi-induced apoptosis in human gastric cancer cell line BGC823. METHODS: Growth inhibition by allitridi was analyzed using cell growth curve and MTT assay. Apoptotic cells were detected using staining with Hoechst 33342, and confirmed by flow cytometric analysis and DNA fragmentation analysis. The protein expression affected by allitridi was determined using Western blot. The activity of caspase-3 was measured using a fluorescence assay. RESULTS: Allitridi induced apoptosis, and then inhibited cells proliferation in human gastric cancer cell line BGC823. The protein level of Bcl-2 was decreased dramatically, while Bax and p53 were not significantly affected by allitridi. The expression and activity of caspase-3 started to increase after allitridi treatment for 72 h. CONCLUSION: Allitridi induced apoptosis through down-regulation of Bcl-2, and increased caspase-3 expression and its activity.

引用

页码：219 / 225

页数：7

共 42 条

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