Transformed tobacco plants with increased tolerance to drought

被引：37

作者：

Gubis, J. ^{[1
]}

Vankova, R. ^{[2
]}

Cervena, V. ^{[1
]}

Dragunova, M. ^{[3
]}

Hudcovicova, M. ^{[1
]}

Lichtnerovia, H. ^{[3
]}

Dokupil, T. ^{[3
]}

Jurekova, Z. ^{[4
]}

机构：

[1] Slovak Agr Res Ctr, Res Inst Plant Product, Piest Any 92168, Slovakia

[2] ASCR, Inst Expt Bot, Lab Hormonal Regulat Plants, Prague 16502 6, Czech Republic

[3] Slovak Univ Agr, Lab Explantate Cultures, Horticulture & Landscape Engn Fac, Nitra 94676, Slovakia

[4] Slovak Univ Agr, Dept Ecol, Fac European Studies & Reg Dev, Nitra 94976, Slovakia

来源：

SOUTH AFRICAN JOURNAL OF BOTANY | 2007年 / 73卷 / 04期

关键词：

Nicotiana tabacum L; pigment; proline; relative water content; stress;

D O I：

10.1016/j.sajb.2007.03.011

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

P5CSF129A cDNA and the nptII marker gene were used for tobacco (Nicotiana tabacum L. cv. Bel B and cv. M51) transformation via Agrobacterium tumefaciens strain LBA4404. Twenty transformed tobacco plants were obtained after transformation of leaf discs. Presence of the transgene was confirmed by polymerase chain reaction (PCR) analysis. Physiological responses to water stress were compared in transgenic and wild-type tobacco plants. Transgenic plants of both cultivars accumulated high levels of free proline. They did not exhibit dry mass relocation or chlorophyll content reduction. Neither precocious senescence, nor leaf necrosis or morphological changes were observed in control and stress conditions (RWC decrease by 7-8%). Transgenic plants with elevated accumulation of osmoprotectants seem to be better adapted to water stress, providing a perspective for future research of stress effects that have a principle role in the functional activity of plants. This study confirmed P5CSF129A to be a candidate gene in crop engineering for enhanced water stress tolerance. (c) 2007 SAAB. Published by Elsevier B.V. All rights reserved.

引用

页码：505 / 511

页数：7

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