Yeast cell surface display of bacterial PET hydrolase as a sustainable biocatalyst for the degradation of polyethylene terephthalate

被引：11
作者：
Chen, Zhuozhi [1 ]
Xiao, Yunjie [1 ]
Weber, Gert [2 ]
Wei, Ren [3 ]
Wang, Zefang [1 ]
机构：
[1] Tianjin Univ, Sch Life Sci, Tianjin, Peoples R China
[2] Helmholtz Zentrum Berlin, Macromol Crystallog, Berlin, Germany
[3] Univ Greifswald, Inst Biochem, Jr Res Grp Plast Biodegradat, Greifswald, Germany
来源：
ENZYMATIC PLASTIC DEGRADATION | 2021年 / 648卷
基金：
中国国家自然科学基金;
关键词：
SACCHAROMYCES-CEREVISIAE; LIPASE; HYDROLYSIS; ANCHOR; POLY(ETHYLENE-TEREPHTHALATE); EXPRESSION; CUTINASE; ENZYMES; OPRF;
D O I：
10.1016/bs.mie.2020.12.030
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Enzymatic hydrolysis of polyethylene terephthalate (PET) is considered to be an environmentally friendly method for the recycling of plastic waste. Recently, a bacterial enzyme named IsPETase was found in Ideonella sakaiensis with the ability to degrade amorphous PET at ambient temperature suggesting its possible use in recycling of PET. However, applying the purified IsPETase in large-scale PET recycling has limitations, i.e., a complicated production process, high cost of single-use, and instability of the enzyme. Yeast cell surface display has proven to be an effectual alternative for improving enzyme degradation efficiency and realizing industrial applications. This chapter deals with the construction and application of a whole-cell biocatalyst by displaying IsPETase on the surface of yeast (Pichia pastoris) cells.
引用
收藏
页码：457 / 477
页数：21
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