Mass spectral compatibility of four proteomics stains

被引：17
作者：
Ball, Malcolm S.
Karuso, Peter [1 ]
机构：
[1] Macquarie Univ, Dept Chem & Biomol Sci, Sydney, NSW 2109, Australia
[2] Macquarie Univ, FLUOROtech Pty Ltd, Sydney, NSW 2109, Australia
来源：
JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 11期
关键词：
peptide mass fingerprinting; epicocconone; deep purple; LavaPurple; krypton; SYPRO ruby; flamingo; fluorescence; total protein stain;
D O I：
10.1021/pr070398z
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
With the recent introduction of new fluorescence stains to the proteomics market, there is now more choice available. SYPRO Ruby, LavaPurple, Flamingo, and Krypton total protein stains were compared for ease of use, image quality, and compatibility with protein identification by peptide mass fingerprinting (PMF) (MALDI-TOF). All four stains produced good images but with slightly different staining patterns. SYPRO was found to inhibit identification of cysteine and tryptophan containing peptides, which reduced protein identification.
引用
收藏
页码：4313 / 4320
页数：8
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