Advanced fluorescence correlation spectroscopy for studying biomolecular conformation

被引：13
作者：
Ghosh, Arindam [1 ]
Enderlein, Joerg [1 ,2 ]
机构：
[1] Univ Gottingen, Inst Phys 3, Friedrich Hund Pl 1, D-37077 Gottingen, Germany
[2] Georg August Univ, Cluster Excellence Multiscale Bioimaging Mol Mach, D-37077 Gottingen, Germany
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2021年 / 70卷
基金：
欧盟地平线“2020”; 欧洲研究理事会;
关键词：
SINGLE-MOLECULE FRET; CROSS-CORRELATION SPECTROSCOPY; ROTATIONAL DIFFUSION; CHAIN MOTIONS; DYNAMICS; PROTEIN; MACROMOLECULES; BINDING; VIEW; DYES;
D O I：
10.1016/j.sbi.2021.06.018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We present the recent developments and advances in fluorescence correlation spectroscopy (FCS) and their application to the investigation of biomolecular conformations. In particular, we present and discuss three techniques: multichannel nanosecond FCS, photo-induced electron transfer FCS, and fluorescence lifetime correlation spectroscopy. We briefly describe each method and discuss recent applications to diverse biophysical studies of biomolecular conformation.
引用
收藏
页码：123 / 131
页数：9
相关论文
共 61 条
  • [11] Fluorescence lifetime correlation spectroscopy: Basics and applications
    Ghosh, Arindam
    Karedla, Narain
    Thiele, Jan Christoph
    Gregor, Ingo
    Enderlein, Jorg
    [J]. METHODS, 2018, 140 : 32 - 39
  • [12] Quantifying Microsecond Transition Times Using Fluorescence Lifetime Correlation Spectroscopy
    Ghosh, Arindam
    Isbaner, Sebastian
    Veiga-Gutierrez, Manoel
    Gregor, Ingo
    Enderlein, Joerg
    Karedla, Narain
    [J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2017, 8 (24): : 6022 - 6028
  • [13] Microsecond sub-domain motions and the folding and misfolding of the mouse prion protein
    Goluguri, Rama Reddy
    Sen, Sreemantee
    Udgaonkar, Jayant
    [J]. ELIFE, 2019, 8
  • [14] Fluorescence Lifetime Correlation Spectroscopy Reveals Compaction Mechanism of 10 and 49 kbp DNA and Differences between Polycation and Cationic Surfactant
    Humpolickova, Jana
    Beranova, Lenka
    Stepanek, Miroslav
    Benda, Ales
    Prochazka, Karel
    Hof, Martin
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2008, 112 (51) : 16823 - 16829
  • [15] Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy. 1. Principle
    Ishii, Kunihiko
    Tahara, Tahei
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2013, 117 (39) : 11414 - 11422
  • [16] Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy. 2. Application
    Ishii, Kunihiko
    Tahara, Tahei
    [J]. JOURNAL OF PHYSICAL CHEMISTRY B, 2013, 117 (39) : 11423 - 11432
  • [17] Jovin T M, 1981, Ann N Y Acad Sci, V366, P176, DOI 10.1111/j.1749-6632.1981.tb20753.x
  • [18] Fluorescent probes and bioconjugation chemistries for single-molecule fluorescence analysis of biomolecules
    Kapanidis, AN
    Weiss, S
    [J]. JOURNAL OF CHEMICAL PHYSICS, 2002, 117 (24) : 10953 - 10964
  • [19] Fluorescence Lifetime Correlation Spectroscopy (FLCS): Concepts, Applications and Outlook
    Kapusta, Peter
    Machan, Radek
    Benda, Ales
    Hof, Martin
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2012, 13 (10) : 12890 - 12910
  • [20] Fluorescence lifetime correlation spectroscopy
    Kapusta, Peter
    Wahl, Michael
    Benda, Ales
    Hof, Martin
    Enderlein, Jorg
    [J]. JOURNAL OF FLUORESCENCE, 2007, 17 (01) : 43 - 48
1234567