Advanced fluorescence correlation spectroscopy for studying biomolecular conformation

被引：13
作者：
Ghosh, Arindam [1 ]
Enderlein, Joerg [1 ,2 ]
机构：
[1] Univ Gottingen, Inst Phys 3, Friedrich Hund Pl 1, D-37077 Gottingen, Germany
[2] Georg August Univ, Cluster Excellence Multiscale Bioimaging Mol Mach, D-37077 Gottingen, Germany
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2021年 / 70卷
基金：
欧盟地平线“2020”; 欧洲研究理事会;
关键词：
SINGLE-MOLECULE FRET; CROSS-CORRELATION SPECTROSCOPY; ROTATIONAL DIFFUSION; CHAIN MOTIONS; DYNAMICS; PROTEIN; MACROMOLECULES; BINDING; VIEW; DYES;
D O I：
10.1016/j.sbi.2021.06.018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We present the recent developments and advances in fluorescence correlation spectroscopy (FCS) and their application to the investigation of biomolecular conformations. In particular, we present and discuss three techniques: multichannel nanosecond FCS, photo-induced electron transfer FCS, and fluorescence lifetime correlation spectroscopy. We briefly describe each method and discuss recent applications to diverse biophysical studies of biomolecular conformation.
引用
收藏
页码：123 / 131
页数：9
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