Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin

被引：251

作者：

El Oualid, Farid ^{[1
]}

Merkx, Remco ^{[1
]}

Ekkebus, Reggy ^{[1
]}

Hameed, Dharjath S. ^{[1
]}

Smit, Judith J. ^{[2
]}

de Jong, Annemieke ^{[1
]}

Hilkmann, Henk ^{[1
]}

Sixma, Titia K. ^{[2
]}

Ovaa, Huib ^{[1
]}

机构：

[1] Netherlands Canc Inst, Div Cell Biol, NL-1066 CX Amsterdam, Netherlands

[2] Netherlands Canc Inst, Div Biochem, NL-1066 CX Amsterdam, Netherlands

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 52期

关键词：

chemical ligation; peptides; solid-phase synthesis; synthetic methods; ubiquitin; DEUBIQUITINATING ENZYMES; PROTEIN UBIQUITINATION; PEPTIDE UBIQUITYLATION; CRYSTAL-STRUCTURE; LIGATION; ACTIVATION; DIPEPTIDES; REVEALS; LINKAGE; SYSTEM;

D O I：

10.1002/anie.201005995

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Changing the subject: An efficient linear solid-phase peptide synthesis of ubiquitin (Ub) has been developed. This approach allows the incorporation of desired tags and mutations (see picture; blue denotes a pseudoproline dipeptide, red a dimethoxybenzyl dipeptide) as well as specific C-terminal modification and the construction of all diubiquitin conjugates in high yields and purities in a straightforward manner. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：10149 / 10153

页数：5

共 48 条

[1]

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