Multi-class pesticide analysis in human hair by gas chromatography tandem (triple quadrupole) mass spectrometry with solid phase microextraction and liquid injection

被引：48
作者：
Salquebre, Guillaume [1 ]
Schummer, Claude [1 ,2 ]
Millet, Maurice [2 ]
Briand, Olivier [3 ]
Appenzeller, Brice M. R. [1 ]
机构：
[1] Univ Luxembourg, CRP Sante, Lab Analyt Human Biomonitoring, L-1511 Luxembourg, Luxembourg
[2] Univ Strasbourg, CNRS, Equipe Phys Chim Atmosphere, LMSPC,UMR 4515, F-67084 Strasbourg, France
[3] French Agcy Food Environm & Occupat Hlth & Safety, Risk Assessment Dept, F-94700 Maisons Alfort, France
来源：
ANALYTICA CHIMICA ACTA | 2012年 / 710卷
关键词：
Pesticides; Hair; GC-MS/MS; SPME; Human biomonitoring; Exposure; DETECT FETAL EXPOSURE; RISK-ASSESSMENT; MATERNAL HAIR; INFANT HAIR; CORD BLOOD; MECONIUM; CONTAMINANTS; METABOLITES; POLLUTANTS; MATRICES;
D O I：
10.1016/j.aca.2011.10.029
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for the simultaneous detection and quantification of 22 pesticides from different chemical classes was developed using solid-phase microextraction (SPME) and gas chromatography tandem (triple quadrupole) mass spectrometry. Pesticides were extracted from 50 mg of pulverized hair with acetonitrile. The extract was submitted to two successive steps of direct immersion-SPME at 30 degrees C and 90 degrees C or to a liquid injection without SPME in order to obtain optimized conditions for each of the 22 analytes investigated. Validation parameters were significantly influenced by both the injection mode (SPME vs liquid injection) and the temperature of SPME. Limits of quantification ranged from 0.05 pg mg(-1) for tri-fluralin to 10 pg mg(-1) for pentachlorophenol. The application of the validated method to the analysis of samples collected from non-occupationally exposed volunteers demonstrated the presence of pesticides in all the samples tested. Altogether, 13 different analytes were detected at concentration above the limit of quantification. (C) 2011 Elsevier B.V. All rights reserved.
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页码：65 / 74
页数：10
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