Effects of polysaccharides from abalone viscera (Haliotis discus hannai Ino) on MGC 803 cells proliferation

被引：20

作者：

Zhang, Rui-Juan ^{[1
]}

Shi, Yan ^{[1
]}

Zheng, Jing ^{[1
]}

Mao, Xiao-Mei ^{[1
]}

Liu, Zhi-Yu ^{[2
]}

Chen, Qing-Xi ^{[1
]}

Wang, Qin ^{[1
]}

机构：

[1] Xiamen Univ, Sch Life Sci, Minist Educ Coastal & Wetland Ecosyst, Key Lab, Xiamen 361102, Peoples R China

[2] Fisheries Reasearch Inst Fujian, Xiamen 361013, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 106卷

关键词：

Abalone viscera; Polysaccharide; MGC; 803; cells;

D O I：

10.1016/j.ijbiomac.2017.08.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The polysaccharides (AVP) was obtained from abalone (Haliotis discus hannai Ino) viscera, using the alkaline protease to enzymolysis, sevage method and repeated freezing and thawing method to remove protein and hydrogen peroxide method to depigment. The total sugar content was 46.27 +/- 1.5% and uronic acid, sulfate radical, hexosamine and protein contents were 17.44 +/- 0.22%, 16.98 +/- 0.15%, 0.65 +/- 0.02% and 1.64 +/- 0.13% in AVP respectively. The main monosaccharide compositions of AVP were D-galactose, D-xylose, D-mannose, D-glucose and D-glucuronic acid. MTT assay showed AVP had a significant anti-tumor activity to gastric carcinoma cells, especially to MGC 803, while it had no influence upon proliferation of normal stomach cells GES 1. The results of Morphological changes, cell migration ability and AO/EB staining indicated that MGC803 cells underwent apoptosis in a dose-dependent manner induced by AVP. Moreover, the western blotting results showed that the expressions of survivin, Bcl-2 and VEGF were decreased, while the expression of Bax and p53 were increased in a dose-dependent manner of AVP. The results suggested that AVP might be a potential anti-tumor agent securely and naturally. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：587 / 595

页数：9