An RBD-Based Diagnostic Method Useful for the Surveillance of Protective Immunity against SARS-CoV-2 in the Population

被引:4
作者
Adriana Ayon-Nunez, Dolores [1 ]
Cervantes-Torres, Jacquelynne [2 ]
Cabello-Gutierrez, Carlos [3 ]
Rosales-Mendoza, Sergio [4 ]
Rios-Valencia, Diana [2 ]
Huerta, Leonor [2 ]
Bobes, Raul J. [2 ]
Cesar Carrero, Julio [2 ]
Segura-Velazquez, Rene [1 ]
Alma Fierro, Nora [2 ]
Hernandez, Marisela [2 ]
Zuniga-Ramos, Joaquin [3 ]
Gamba, Gerardo [2 ,5 ]
Cardenas, Graciela [6 ]
Frias-Jimenez, Emmanuel [7 ]
Alonso Herrera, Luis [7 ]
Fragoso, Gladis [2 ]
Sciutto, Edda [2 ]
Suarez-Guemes, Francisco [1 ]
Pedro Laclette, Juan [2 ]
机构
[1] Univ Nacl Autonoma Mexico, Sch Vet Med & Zootech, Ciudad de Mexico 04510, Mexico
[2] Univ Nacl Autonoma Mexico, Biomed Res Inst, Ciudad de Mexico 04510, Mexico
[3] Inst Nacl Enfermedades Resp Ismael Cosio Villegas, Calzada Tlalpan 4502,Belisario Dominguez Secc 16, Ciudad de Mexico 14080, Mexico
[4] Univ Autonoma San Luis Potosi, Fac Ciencias Quim, Lab Biofarmaceut Recombinantes, Av Dr Manuel Nava 6, San Luis Potosi 78210, San Luis Potosi, Mexico
[5] Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Vasco de Quiroga 15,Belisario Dominguez Secc 16, Ciudad de Mexico 14080, Mexico
[6] Inst Nacl Neurol & Neurocirug, Av Insurgentes Sur 3877, Ciudad de Mexico 14269, Mexico
[7] Inst Nacl Med Genom, Perifer Sur 4809, Ciudad de Mexico 14610, Mexico
关键词
COVID-19; receptor-binding domain (RBD); serologic diagnosis; ELISA; neutralizing antibodies; RECEPTOR-BINDING-DOMAIN; ANTIBODIES;
D O I
10.3390/diagnostics12071629
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
After more than two years, the COVID-19 pandemic is still ongoing and evolving all over the world; human herd immunity against SARS-CoV-2 increases either by infection or by unprecedented mass vaccination. A substantial change in population immunity is expected to contribute to the control of transmission. It is essential to monitor the extension and duration of the population's immunity to support the decisions of health authorities in each region and country, directed to chart the progressive return to normality. For this purpose, the availability of simple and cheap methods to monitor the levels of relevant antibodies in the population is a widespread necessity. Here, we describe the development of an RBD-based ELISA for the detection of specific antibodies in large numbers of samples. The recombinant expression of an RBD-poly-His fragment was carried out using either bacterial or eukaryotic cells in in vitro culture. After affinity chromatography purification, the performance of both recombinant products was compared by ELISA in similar trials. Our results showed that eukaryotic RBD increased the sensitivity of the assay. Interestingly, our results also support a correlation of the eukaryotic RBD-based ELISA with other assays aimed to test for neutralizing antibodies, which suggests that it provides an indication of protective immunity against SARS-CoV-2.
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页数:11
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