Viable but Non-culturable State of Bacterial Cold-water Disease Pathogen Flavobacterium psychrophilum at Various Temperatures

被引：8

作者：

Sugahara, Kazuhiro ^{[1
,2
]}

Fujiwara-Nagata, Erina ^{[3
]}

Fukuda, Ayami ^{[2
]}

Eguchi, Mitsuru ^{[3
]}

机构：

[1] Shiga Prefectural Fisheries Expt Stn, Shiga 5220057, Japan

[2] Kinki Univ, Grad Sch Agr, Nara 6318505, Japan

[3] Kinki Univ, Dept Fisheries, Nara 6318505, Japan

来源：

FISH PATHOLOGY | 2010年 / 45卷 / 04期

关键词：

viable but non-culturable; Flavobacterium psychrophilum; bacterial cold-water disease; Plecoglossus altivelis; VBNC; BCWD; CYTOPHAGA-PSYCHROPHILA; VIBRIO-CHOLERAE; SURVIVAL; ENVIRONMENT; STARVATION; OUTBREAKS; SEAWATER; STRAINS; AYU;

D O I：

10.3147/jsfp.45.158

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Bacterial cold-water disease (BCWD) caused by Flavobacterium psychrophilum leads to heavy mortality of ayu Plecoglossus altivelis in Japan. Previously, a 28 degrees C warmed water treatment was shown to be an effective treatment for BCWD, since F psychrophilum can not grow and maintain colonies at this temperature. However, it was unclear whether the bacteria might resuscitate after the treatment was over. Therefore, we investigated colony formation, membrane potential and pathogenicity of F psychrophilum at various temperatures (15 degrees C, 23 degrees C, 28 degrees C and 33 degrees C) in sterilized underground water. Within 2 days at 28 degrees C, F psychrophilum completely lost their colony-forming abilities but still maintained their membrane potentials. It seemed that these cells entered into viable but non-culturable (VBNC) state. However, experimental ayu infection revealed that VBNC F psychrophilum cells were unable to cause BCWD, suggesting that the cells were progressing towards death at 28 degrees C.

引用

页码：158 / 163

页数：6

共 27 条

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