JCB-SGT Crystallization Devices Applicable to PCG Experiments and their Crystallization Conditions

被引：12

作者：

Takahashi, Sachiko ^{[1
]}

Koga, Misako ^{[1
]}

Yan, Bin ^{[1
]}

Furubayashi, Naoki ^{[2
]}

Kamo, Masayuki ^{[2
]}

Inaka, Koji ^{[2
]}

Tanaka, Hiroaki ^{[1
]}

机构：

[1] Confocal Sci Inc, Chiyoda Ku, 7F Hayakawa 2nd Bldg,2-12-2 Iwamoto Cho, Tokyo 1010032, Japan

[2] Maruwa Foods & Biosci Inc, 170-1 Tsutsui Cho, Yamato Koriyama, Nara 6391123, Japan

来源：

INTERNATIONAL JOURNAL OF MICROGRAVITY SCIENCE AND APPLICATION | 2019年 / 36卷 / 01期

关键词：

Protein crystallization; JCB-SGT; Crystallization device; Counter-diffusion; Vapor-diffusion; Batch; Dialysis; Osmosis; Space experiment; Microgravity; JAXA; PROTEIN CRYSTALLIZATION; DIFFUSION;

D O I：

10.15011//jasma.36.360107

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

JCB-SGT, which is the primary crystallization container used in JAXA PCG (Protein Crystal Growth) experiments, is simple, versatile and has a wide range of applications. Various crystallization methods can be applied in JCB-SGT: counter-diffusion method, dialysis method, vapor-diffusion method, osmosis-tube method and batch method. We have supported users of JAXA PCG for nearly 20 years and incrementally improved our technologies to obtain high-quality protein crystals in space. In this review, the outline of JCB-SGT is described and important insights and lessons learned from our experiences in optimizing crystallization conditions are explained. These are helpful for PCG users to optimize their protein samples for experimentation, contributing to both space-based experiments and also to laboratory experiments on the ground.

引用

页数：9

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