Fluorescence Nanoscopy of Single DNA Molecules by Using Stimulated Emission Depletion (STED)

被引：44
|
作者：
Persson, F. [2 ]
Bingen, P. [1 ]
Staudt, T. [1 ]
Engelhardt, J. [1 ]
Tegenfeldt, J. O. [2 ]
Hell, Stefan W. [1 ]
机构：
[1] German Canc Res Ctr, Opt Nanoscopy Div, D-69120 Heidelberg, Germany
[2] Univ Gothenburg, Dept Phys, S-41296 Gothenburg, Sweden
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 24期
基金：
瑞典研究理事会;
关键词：
DNA; dyes/pigments; fluorescence; single-molecule studies; stimulated emission depletion (STED); LIMIT;
D O I：
10.1002/anie.201100371
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Clearing the haze: Stimulated emission depletion (STED) nanoscopy on single DNA strands reveals details with an up to five- to sixfold improved resolution over confocal microscopy (see images). Thus STED nanoscopy allows features to be distinguished down to the persistence length of DNA (ca. 50nm) without promoting any significant additional photodamage, such as photobleaching or photonicking. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：5581 / 5583
页数：3
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