Regulators of G protein signaling (RGS) proteins constitutively activate Gβγ-gated potassium channels

被引：60

作者：

Bünemann, M ^{[1
]}

Hosey, MM ^{[1
]}

机构：

[1] Northwestern Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Chicago, IL 60611 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1998年 / 273卷 / 47期

关键词：

D O I：

10.1074/jbc.273.47.31186

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report novel effects of regulators of G protein signaling (RGS) on G protein-regulated ion channels. RGS3 and RGS4 induced a substantial increase in currents through the G beta gamma-regulated inwardly rectifying K+ channels, I-K(ACh), in the absence of receptor activation. Concomitantly, the amount of current that could be activated by agonist was reduced. Pretreatment with pertussis toxin or a muscarinic receptor antagonist abolished agonist-induced currents but did not modify RGS effects. Cotransfection of cells with a G beta gamma-binding protein significantly reduced the RGS4-induced basal I-K(ACh) currents. The RGS proteins also modified the properties of another G beta gamma effector, the N-type Ca2+ channels. These observations strongly suggest that RGS proteins increase the availability of G beta gamma in addition to their previously described GTPase-activating function.

引用

页码：31186 / 31190

页数：5

共 45 条

[11] ATRIAL G-PROTEIN-ACTIVATED K+-CHANNEL - EXPRESSION CLONING AND MOLECULAR-PROPERTIES [J].

DASCAL, N ;

SCHREIBMAYER, W ;

LIM, NF ;

WANG, WZ ;

CHAVKIN, C ;

DIMAGNO, L ;

LABARCA, C ;

KIEFFER, BL ;

GAVERIAUXRUFF, C ;

TROLLINGER, D ;

LESTER, HA ;

DAVIDSON, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) :10235-10239

[12] Direct binding of G-protein beta gamma complex to voltage-dependent calcium channels [J].

DeWaard, M ;

Liu, HY ;

Walker, D ;

Scott, VES ;

Gurnett, CA ;

Campbell, KP .

NATURE, 1997, 385 (6615) :446-450

[13]

DOHLMAN HG, 1995, MOL CELL BIOL, V15, P3635

[14] RGS proteins reconstitute the rapid gating kinetics of G beta gamma-activated inwardly rectifying K+ channels [J].

Doupnik, CA ;

Davidson, N ;

Lester, HA ;

Kofuji, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (19) :10461-10466

[15] Inhibition of C-protein-mediated MAP kinase activation by a new mammalian gene family [J].

Druey, KM ;

Blumer, KJ ;

Kang, VH ;

Kehrl, JH .

NATURE, 1996, 379 (6567) :742-746

[16] The core domain of a new retina specific RGS protein stimulates the GTPase activity of transducin in vitro [J].

Faurobert, E ;

Hurley, JB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (07) :2945-2950

[17] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[18] RGS9, a GTPase accelerator for phototransduction [J].

He, W ;

Cowan, CW ;

Wensel, TG .

NEURON, 1998, 20 (01) :95-102

[19] RGS4 and GAIP are GTPase-activating proteins for G(q alpha) and block activation of phospholipase C beta by gamma-thio-GTP-G(q alpha) [J].

Hepler, JR ;

Berman, DM ;

Gilman, AG ;

Kozasa, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (02) :428-432

[20] Modulation of Ca2+ channels by G-protein beta gamma subunits [J].

Herlitze, S ;

Garcia, DE ;

Mackie, K ;

Hille, B ;

Scheuer, T ;

Catterall, WA .

NATURE, 1996, 380 (6571) :258-262

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