Chemoenzymatic Probes for Detecting and Imaging Fucose-α(1-2)-galactose Glycan Biomarkers
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作者:
Chaubard, Jean-Luc
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CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USACALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
Chaubard, Jean-Luc
[1
,2
]
Krishnamurthy, Chithra
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CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USACALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
Krishnamurthy, Chithra
[1
,2
]
Yi, Wen
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机构:
CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USACALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
Yi, Wen
[1
,2
]
Smith, David F.
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Emory Univ, Dept Biochem, Sch Med, Atlanta, GA 30322 USA
Emory Univ, Glyc Ctr, Sch Med, Atlanta, GA 30322 USACALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
Smith, David F.
[3
,4
]
Hsieh-Wilson, Linda C.
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机构:
CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USACALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
Hsieh-Wilson, Linda C.
[1
,2
]
机构:
[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
[2] CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA
[3] Emory Univ, Dept Biochem, Sch Med, Atlanta, GA 30322 USA
[4] Emory Univ, Glyc Ctr, Sch Med, Atlanta, GA 30322 USA
The disaccharide motif fucose-alpha(1-2)-galactose (Fuc alpha(1-2)Gal) is involved in many important physiological processes, such as learning and memory, inflammation, asthma, and tumorigenesis. However, the size and structural complexity of Fuc alpha(1-2) Gal-containing glycans have posed a significant challenge to their detection. We report a new chemoenzymatic strategy for the rapid, sensitive detection of Fuc alpha(1-2)Gal glycans. We demonstrate that the approach is highly selective for the Fuc alpha(1-2)Gal motif, detects a variety of complex glycans and glycoproteins, and can be used to profile the relative abundance of the motif on live cells, discriminating malignant from normal cells. This approach represents a new potential strategy for biomarker detection and expands the technologies available for understanding the roles of this important class of carbohydrates in physiology and disease.