Chemoenzymatic Probes for Detecting and Imaging Fucose-α(1-2)-galactose Glycan Biomarkers

被引:76
作者
Chaubard, Jean-Luc [1 ,2 ]
Krishnamurthy, Chithra [1 ,2 ]
Yi, Wen [1 ,2 ]
Smith, David F. [3 ,4 ]
Hsieh-Wilson, Linda C. [1 ,2 ]
机构
[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
[2] CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA
[3] Emory Univ, Dept Biochem, Sch Med, Atlanta, GA 30322 USA
[4] Emory Univ, Glyc Ctr, Sch Med, Atlanta, GA 30322 USA
关键词
ALTERED GLYCOSYLATION; EXPRESSION; CANCER; PROTEIN; ANTIGENS; FUCOSE; LUNG;
D O I
10.1021/ja211312u
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The disaccharide motif fucose-alpha(1-2)-galactose (Fuc alpha(1-2)Gal) is involved in many important physiological processes, such as learning and memory, inflammation, asthma, and tumorigenesis. However, the size and structural complexity of Fuc alpha(1-2) Gal-containing glycans have posed a significant challenge to their detection. We report a new chemoenzymatic strategy for the rapid, sensitive detection of Fuc alpha(1-2)Gal glycans. We demonstrate that the approach is highly selective for the Fuc alpha(1-2)Gal motif, detects a variety of complex glycans and glycoproteins, and can be used to profile the relative abundance of the motif on live cells, discriminating malignant from normal cells. This approach represents a new potential strategy for biomarker detection and expands the technologies available for understanding the roles of this important class of carbohydrates in physiology and disease.
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页码:4489 / 4492
页数:4
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