Antioxidant enzyme activities of human peripheral blood mononuclear cells exposed to trace elements

被引:21
作者
Kuppusamy, UR [1 ]
Dharmani, M [1 ]
Kanthimathi, MS [1 ]
Indran, M [1 ]
机构
[1] Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur 50603, Malaysia
关键词
antioxidant enzymes; trace elements; mononuclear cells;
D O I
10.1385/BTER:106:1:029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The trace elements copper, zinc, and selenium are important immune modulators and essential cofactors of the antioxidant enzymes. In the present study, the proliferative effect of human peripheral mononuclear cells (PBMCs) that have been exposed to copper, zinc, and selenium and the corresponding activities of antioxidant enzymes, namely superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase, were determined. Zinc and copper stimulated the PBMC proliferation in a dose-dependent manner within the dose range 25-200 mu mol/L. SOD and GPx activities in PBMCs exposed to zinc were inhibited, whereas catalase activity was unaffected. All the three antioxidant enzymes in the cells exposed to copper were inhibited. Selenium exerted more potent inhibition of the cell proliferation while causing stimulation of the antioxidant enzymes at the lowest dose (25 mu mol/L) than at the highest dose (200 mu mol/L) tested. A significant negative correlation was observed between proliferation and antioxidant enzyme (SOD and GPx) activities in trace-element-exposed PBMC. The present findings substantiate the importance of trace elements as immune modulators and the involvement of enzymatic antioxidant system in the immune cell regulation.
引用
收藏
页码:29 / 39
页数:11
相关论文
共 50 条
[21]   The effects of heparin on the adhesion of human peripheral blood mononuclear cells to human stimulated umbilical vein endothelial cells [J].
Smailbegovic, A ;
Lever, R ;
Page, CP .
BRITISH JOURNAL OF PHARMACOLOGY, 2001, 134 (04) :827-836
[22]   The effect of diethyldithiocarbamate on antioxidant enzyme activities in the blood of rats [J].
Radojicic, R ;
Borkovic, S ;
Pavlovic, S ;
Nikolic, A ;
Blagojevic, D ;
Saicic, Z ;
Spasic, M .
ACTA VETERINARIA-BEOGRAD, 2002, 52 (5-6) :329-335
[23]   Antioxidant status and glutathione metabolism in peripheral blood mononuclear cells from patients with chronic hepatitis C [J].
Boya, P ;
de la Peña, A ;
Beloqui, O ;
Larrea, E ;
Conchillo, M ;
Castelruiz, Y ;
Civeira, MP ;
Prieto, J .
JOURNAL OF HEPATOLOGY, 1999, 31 (05) :808-814
[24]   Glucocorticoid receptors in human peripheral blood mononuclear cells in relation to age and to sport activity [J].
Grasso, G ;
Lodi, L ;
Lupo, C ;
Muscettola, M .
LIFE SCIENCES, 1997, 61 (03) :301-308
[25]   Cytokine production by human peripheral blood mononuclear cells: Differential sensitivity to glutamine availability [J].
Yaqoob, P ;
Calder, PC .
CYTOKINE, 1998, 10 (10) :790-794
[26]   Trace elements exposure influences proximate body composition and antioxidant enzyme activities of the species tilapia and catfish in Burullus Lake—Egypt: human risk assessment for the consumers [J].
Heba H. Abdel-Kader ;
Mohamed H. Mourad .
Environmental Science and Pollution Research, 2020, 27 :43670-43681
[27]   A method for production and determination of histamine releasing activity from human peripheral blood mononuclear cells [J].
Kampen, GT ;
Poulsen, LK ;
Reimert, CM ;
Skov, PS .
JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 210 (02) :185-193
[28]   In vitro effect of statins on cytokine production and mitogen response of human peripheral blood mononuclear cells [J].
Bessler, H ;
Salman, H ;
Bergman, M ;
Straussberg, R ;
Djaldetti, M .
CLINICAL IMMUNOLOGY, 2005, 117 (01) :73-77
[29]   Cytokine secretion from human peripheral blood mononuclear cells cultured in vitro with metal particles [J].
Cachinho, Sandra C. P. ;
Pu, Fanrong ;
Hunt, John A. .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2013, 101 (04) :1201-1209
[30]   Expression levels of seven candidate genes in human peripheral blood mononuclear cells and their association with preeclampsia [J].
Martinez-Fierro, M. L. ;
Garza-Veloz, I. ;
Carrillo-Sanchez, K. ;
Martinez-Gaytan, V. ;
Cortes-Flores, R. ;
Ochoa-Torres, M. A. ;
Guerrero, G. G. ;
Rodriguez-Sanchez, I. P. ;
Cancela-Murrieta, C. O. ;
Zamudio-Osuna, M. ;
Badillo-Almaraz, J. I. ;
Castruita-De la Rosa, C. .
HYPERTENSION IN PREGNANCY, 2014, 33 (02) :191-203