Resveratrol Upregulated SIRT1, FOXO1, and Adiponectin and Downregulated PPARγ1-3 mRNA Expression in Human Visceral Adipocytes

被引：69

作者：

Costa, Cintia dos Santos ^{[1
]}

Rohden, Francieli ^{[1
]}

Hammes, Thais Ortiz ^{[1
]}

Margis, Rogerio ^{[1
,2
]}

Bortolotto, Josiane Woutheres ^{[1
]}

Padoin, Alexandre Vontobel ^{[3
]}

Mottin, Claudio Cora ^{[3
]}

Guaragna, Regina Maria ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, ICBS, Dept Bioquim, BR-90035003 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Ctr Biotecnol, BR-90035003 Porto Alegre, RS, Brazil

[3] Hosp Sao Lucas PUCRS, Ctr Obesidade & Sindrome Metab, Porto Alegre, RS, Brazil

来源：

OBESITY SURGERY | 2011年 / 21卷 / 03期

关键词：

Adipocytes; SIRT1; FOXO1; Adiponectin; PPAR gamma 1-3; Resveratrol; ACTIVATED RECEPTOR-GAMMA; GENE-EXPRESSION; METABOLISM; MODULATION;

D O I：

10.1007/s11695-010-0251-7

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

The SIRT1 enzyme is involved in adipose tissue (AT) lipolysis. FOXO1 is a protein that plays a significant role in regulating metabolism. Adiponectin is an adipokine, secreted by the AT, which has been considered to have an antiobesity function. PPAR gamma is one of the key actors in adipocytes differentiation. This study was undertaken to investigate whether resveratrol can regulate SIRT1, FOXO1, adiponectin, PPAR gamma 1-3, and PPAR beta/delta in human AT. The effects of resveratrol were analyzed in freshly isolated adipocytes prepared from visceral fat tissue samples obtained during bariatric surgery. Genes messenger ribonucleic acid (mRNA) levels were determined by qRT-PCR. Ours results show that resveratrol modulates the studied genes, increasing SIRT1 (p = 0.021), FOXO1 (p = 0.001), and adiponectin (p = 0.025) mRNA expression and decreasing PPAR gamma 1-3 (p = 0.003) mRNA in human visceral adipocytes. Resveratrol, in vitro and at low concentration, modulates genes that are related to lipid metabolism, possibly preventing metabolic disease in human visceral adipose tissue (VAT).

引用

页码：356 / 361

页数：6

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