A ubiquitin-like peptide with ribonuclease activity against various polyhomoribonucleotides from the yellow mushroom Cantharellus cibarius

被引：28

作者：

Wang, HX

Ngai, HK

Ng, TB ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Fac Med, Shatin, NT, Peoples R China

[2] China Agr Univ, Dept Microbiol, Coll Biol Sci, Beijing, Peoples R China

来源：

PEPTIDES | 2003年 / 24卷 / 04期

关键词：

ubiquitin; mushroom; ribonuclease; Cantharellus cibarius;

D O I：

10.1016/S0196-9781(03)00116-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A peptide, with a molecular weight of 9 K and an N-terminal sequence identical to that of ubiquitin, was isolated from fresh fruiting bodies of the yellow mushroom Cantharellus cibarius. The peptide manifested ribonucleolytic activity toward poly A, poly C, poly G, and poly U, with the activity toward the first two polyhomoribonucleotides being higher. Maximal activity toward yeast tRNA was observed at a temperature of 70degreesC and a pH of 7. The peptide was adsorbed on DEAE-cellulose, CM-Sepharose, and Q-Sepharose. The yield of the peptide was 7 mg from 1.8 kg mushroom. (C) 2003 Elsevier Science Inc. All rights reserved.

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收藏

页码：509 / 513

页数：5

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