A novel approach to identify proteins modified by nitric oxide: the HIS-TAG switch method

被引：68
作者：
Camerini, Serena
Polci, Maria L.
Restuccia, Umberto
Usuelli, Vera
Malgaroli, Antonio
Bachi, Angela
机构：
[1] Ist Sci San Raffaele, DIBIT, Mass Spectrometry Unit, I-20132 Milan, Italy
[2] Univ Vita Salute San Raffaele, I-20132 Milan, Italy
来源：
JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 08期
关键词：
S-nitrosylation; proteomics; mass spectrometry; post-translational modifications; nitric oxide;
D O I：
10.1021/pr0701456
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
S-nitrosylation is emerging as an important signaling mechanism that regulates a broad range of cellular functions. The recognition of Cysteine residues that undergo S-nitrosylation is crucial to elucidate how this modification modulates protein activity. We report here a novel strategy, defined His-tag switch, which allows the purification and identification of S-nitrosylated proteins and the unambiguous localization of the modified cysteine residues by mass spectrometry analysis.
引用
收藏
页码：3224 / 3231
页数：8
相关论文
共 52 条
[41]   Nitric oxide inhibits an interaction between JNK1 and c-Jun through nitrosylation [J].
Park, Hee-Sae ;
Mo, Jung-Soon ;
Choi, Eui-Ju .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 351 (01) :281-286
[42]   A REQUIREMENT FOR THE INTERCELLULAR MESSENGER NITRIC-OXIDE IN LONG-TERM POTENTIATION [J].
SCHUMAN, EM ;
MADISON, DV .
SCIENCE, 1991, 254 (5037) :1503-1506
[43]   Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels [J].
Shevchenko, A ;
Wilm, M ;
Vorm, O ;
Mann, M .
ANALYTICAL CHEMISTRY, 1996, 68 (05) :850-858
[44]   S-nitrosylation of NSF controls membrane trafficking [J].
Söllner, TH ;
Sequeira, S .
CELL, 2003, 115 (02) :127-129
[45]   (S)NO signals: Translocation, regulation, and a consensus motif [J].
Stamler, JS ;
Toone, EJ ;
Lipton, SA ;
Sucher, NJ .
NEURON, 1997, 18 (05) :691-696
[46]   Studying the S-nitrosylation of model peptides and eNOS protein by mass spectrometry [J].
Taldone, FS ;
Tummala, M ;
Goldstein, EJ ;
Ryzhov, V ;
Ravi, K ;
Black, SM .
NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 2005, 13 (03) :176-187
[47]   S-Nitrosylated protein-disulphide isomerase links protein misfolding to neurodegeneration [J].
Uehara, Takashi ;
Nakamura, Tomohiro ;
Yao, Dongdong ;
Shi, Zhong-Qing ;
Gu, Zezong ;
Ma, Yuliang ;
Masliah, Eliezer ;
Nomura, Yasuyuki ;
Lipton, Stuart A. .
NATURE, 2006, 441 (7092) :513-517
[48]   Nitric oxide protects against mitochondrial permeabilization induced by glutathione depletion: Role of S-nitrosylation? [J].
Whiteman, M ;
Chua, YL ;
Zhang, DW ;
Duan, W ;
Liou, YC ;
Armstrong, JS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 339 (01) :255-262
[49]   Structural and biochemical studies of p21Ras S-nitrosylation and nitric oxide-mediated guanine nucleotide exchange [J].
Williams, JG ;
Pappu, K ;
Campbell, SL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) :6376-6381
[50]   INHIBITION OF CYTOCHROMES-P450 BY NITRIC-OXIDE AND A NITRIC OXIDE-RELEASING AGENT [J].
WINK, DA ;
OSAWA, Y ;
DARBYSHIRE, JF ;
JONES, CR ;
ESHENAUR, SC ;
NIMS, RW .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 300 (01) :115-123
123456