A novel approach to identify proteins modified by nitric oxide: the HIS-TAG switch method

被引：67
作者：
Camerini, Serena
Polci, Maria L.
Restuccia, Umberto
Usuelli, Vera
Malgaroli, Antonio
Bachi, Angela
机构：
[1] Ist Sci San Raffaele, DIBIT, Mass Spectrometry Unit, I-20132 Milan, Italy
[2] Univ Vita Salute San Raffaele, I-20132 Milan, Italy
来源：
JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 08期
关键词：
S-nitrosylation; proteomics; mass spectrometry; post-translational modifications; nitric oxide;
D O I：
10.1021/pr0701456
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
S-nitrosylation is emerging as an important signaling mechanism that regulates a broad range of cellular functions. The recognition of Cysteine residues that undergo S-nitrosylation is crucial to elucidate how this modification modulates protein activity. We report here a novel strategy, defined His-tag switch, which allows the purification and identification of S-nitrosylated proteins and the unambiguous localization of the modified cysteine residues by mass spectrometry analysis.
引用
收藏
页码：3224 / 3231
页数：8
相关论文
共 52 条
[1]   S-nitrosylation of Bcl-2 inhibits its ubiquitin-proteasomal degradation -: A novel antiapoptotic mechanism that suppresses apoptosis [J].
Azad, Neelam ;
Vallyathan, Val ;
Wang, Liying ;
Tantishaiyakul, Vimon ;
Stehlik, Christian ;
Leonard, Stephen S. ;
Rojanasakul, Yon .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (45) :34124-34134
[2]   Inhibition of protein-tyrosine phosphatases by mild oxidative stresses is dependent on S-nitrosylation [J].
Barrett, DM ;
Black, SM ;
Todor, H ;
Schmidt-Ullrich, RK ;
Dawson, KS ;
Mikkelsen, RB .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (15) :14453-14461
[3]   Novel neural modulators [J].
Boehning, D ;
Snyder, SH .
ANNUAL REVIEW OF NEUROSCIENCE, 2003, 26 :105-131
[4]   ISOLATION OF NITRIC-OXIDE SYNTHETASE, A CALMODULIN-REQUIRING ENZYME [J].
BREDT, DS ;
SNYDER, SH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) :682-685
[5]   Direct activation of the olfactory cyclic nucleotide-gated channel through modification of sulfhydryl groups by NO compounds [J].
Broillet, MC ;
Firestein, S .
NEURON, 1996, 16 (02) :377-385
[6]  
Camerini Serena, 2005, Ann Ist Super Sanita, V41, P451
[7]   Guanylate cyclase and the .NO/cGMP signaling pathway [J].
Denninger, JW ;
Marletta, MA .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1999, 1411 (2-3) :334-350
[8]   Reductive assays for S-nitrosothiols:: Implications for measurements in biological systems [J].
Fang, KZ ;
Ragsdale, NV ;
Carey, RM ;
MacDonald, T ;
Gaston, B .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 252 (03) :535-540
[9]   S-nitrosylation in health and disease [J].
Foster, MW ;
McMahon, TJ ;
Stamler, JS .
TRENDS IN MOLECULAR MEDICINE, 2003, 9 (04) :160-168
[10]   THE OBLIGATORY ROLE OF ENDOTHELIAL-CELLS IN THE RELAXATION OF ARTERIAL SMOOTH-MUSCLE BY ACETYLCHOLINE [J].
FURCHGOTT, RF ;
ZAWADZKI, JV .
NATURE, 1980, 288 (5789) :373-376
123456