Modelling glioma invasion using 3D bioprinting and scaffold-free 3D culture
被引:49
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作者:
van Pel, Derek M.
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Univ British Columbia, Dept Cellular & Physiol Sci, Fac Med, Vancouver, BC V6T 1Z3, CanadaUniv British Columbia, Dept Cellular & Physiol Sci, Fac Med, Vancouver, BC V6T 1Z3, Canada
van Pel, Derek M.
[1
]
Harada, Kaori
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Cyfuse Biomed KK, Bunkyo Ku, Univ Tokyo Entrepreneur Plaza,7-3-1 Hongo, Tokyo 1130033, JapanUniv British Columbia, Dept Cellular & Physiol Sci, Fac Med, Vancouver, BC V6T 1Z3, Canada
Harada, Kaori
[2
]
Song, Dandan
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Cyfuse Biomed KK, Bunkyo Ku, Univ Tokyo Entrepreneur Plaza,7-3-1 Hongo, Tokyo 1130033, JapanUniv British Columbia, Dept Cellular & Physiol Sci, Fac Med, Vancouver, BC V6T 1Z3, Canada
Song, Dandan
[2
]
Naus, Christian C.
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Univ British Columbia, Dept Cellular & Physiol Sci, Fac Med, Vancouver, BC V6T 1Z3, CanadaUniv British Columbia, Dept Cellular & Physiol Sci, Fac Med, Vancouver, BC V6T 1Z3, Canada
Naus, Christian C.
[1
]
Sin, Wun Chey
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Univ British Columbia, Dept Cellular & Physiol Sci, Fac Med, Vancouver, BC V6T 1Z3, CanadaUniv British Columbia, Dept Cellular & Physiol Sci, Fac Med, Vancouver, BC V6T 1Z3, Canada
Sin, Wun Chey
[1
]
机构:
[1] Univ British Columbia, Dept Cellular & Physiol Sci, Fac Med, Vancouver, BC V6T 1Z3, Canada
[2] Cyfuse Biomed KK, Bunkyo Ku, Univ Tokyo Entrepreneur Plaza,7-3-1 Hongo, Tokyo 1130033, Japan
3D bioprinting;
Cerebral organoid;
Confocal microscopy;
Connexin43;
Gap junctions;
Glioma;
MICROGLIA-LIKE CELLS;
STEM-CELLS;
BRAIN;
GENERATION;
LANDSCAPE;
D O I:
10.1007/s12079-018-0469-z
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Glioma is a highly aggressive form of brain cancer, with some subtypes having 5-year survival rates of less than 5%. Tumour cell invasion into the surrounding parenchyma seems to be the primary driver of these poor outcomes, as most gliomas recur within 2cm of the original surgically-resected tumour. Many current approaches to the development of anticancer therapy attempt to target genetic weaknesses in a particular cancer, but may not take into account the microenvironment experienced by a tumour and the patient-specific genetic differences in susceptibility to treatment. Here we demonstrate the use of complementary approaches, 3D bioprinting and scaffold-free 3D tissue culture, to examine the invasion of glioma cells into neural-like tissue with 3D confocal microscopy. We found that, while both approaches were successful, the use of 3D tissue culture for organoid development offers the advantage of broad accessibility. As a proof-of-concept of our approach, we developed a system in which we could model the invasion of human glioma cells into mouse neural progenitor cell-derived spheroids. We show that we can follow invasion of human tumour cells using cell-tracking dyes and 3D laser scanning confocal microscopy, both in real time and in fixed samples. We validated these results using conventional cryosectioning. Our scaffold-free 3D approach has broad applicability, as we were easily able to examine invasion using different neural progenitor cell lines, thus mimicking differences that might be observed in patient brain tissue. These results, once applied to iPSC-derived cerebral organoids that incorporate the somatic genetic variability of patients, offer the promise of truly personalized treatments for brain cancer.
机构:
Ecole Natl Super Architecture Paris Malaquais, Lab GSA, 14,Rue Bonaparte 1,Rue Jacques Callot, F-75006 Paris, Ile De France, FranceEcole Natl Super Architecture Paris Malaquais, Lab GSA, 14,Rue Bonaparte 1,Rue Jacques Callot, F-75006 Paris, Ile De France, France
Motamedi, Mahan
Mesnil, Romain
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机构:
Ecole Ponts ParisTech, Lab Navier, 6-8 Ave Blaise Pascal Cite Descartes, F-77455 Champs Sur Marne, Ile De France, FranceEcole Natl Super Architecture Paris Malaquais, Lab GSA, 14,Rue Bonaparte 1,Rue Jacques Callot, F-75006 Paris, Ile De France, France
Mesnil, Romain
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机构:
Oval, Robin
Baverel, Olivier
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机构:
Ecole Natl Super Architecture Paris Malaquais, Lab GSA, 14,Rue Bonaparte 1,Rue Jacques Callot, F-75006 Paris, Ile De France, France
Ecole Ponts ParisTech, Lab Navier, 6-8 Ave Blaise Pascal Cite Descartes, F-77455 Champs Sur Marne, Ile De France, FranceEcole Natl Super Architecture Paris Malaquais, Lab GSA, 14,Rue Bonaparte 1,Rue Jacques Callot, F-75006 Paris, Ile De France, France