RETRACTED: Berberine Attenuates IL-1β-Induced Damage of Nucleus Pulposus Cells via Activating the AMPK/mTOR/Ulk1 Pathway (Retracted Article)

被引：8

作者：

Huang, Liaoyuan ^{[1
]}

Chen, Jianming ^{[1
]}

Wu, Danhai ^{[1
]}

Wang, Kan ^{[2
]}

Lou, Weigang ^{[1
]}

Wu, Jianmin ^{[3
]}

机构：

[1] Ningbo No 6 Hosp, Dept Orthoped, Ningbo 315040, Zhejiang, Peoples R China

[2] Ningbo No 6 Hosp, Dept Radiol Emergency, Ningbo 315040, Zhejiang, Peoples R China

[3] Ningbo No 6 Hosp, Dept Radiol, Ningbo 315040, Zhejiang, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2022年 / 2022卷

关键词：

INTERVERTEBRAL DISC DEGENERATION; MOLECULAR-BASIS; APOPTOSIS; AUTOPHAGY; AMPK; INFLAMMATION; DEGRADATION; KB;

D O I：

10.1155/2022/6133629

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Intervertebral disc degeneration (IDD) is a chronic progressive condition mainly caused by excessive inflammatory cytokines. Berberine (BBR) exerts anti-inflammatory effect on diseases and protective effect against IDD. However, the mechanism is not uncertain. This study is aimed at investigating the molecular mechanism of BBR on IDD. Nucleus pulposus (NP) cells were treated with BBR at different concentrations. The IDD rat model was established by acupuncture. The effect of BBR on interleukin- (IL-) 1 beta-induced cell proliferation was measured by CCK-8 assay and BrdU staining. The role of BBR in IL-1 beta-induced apoptosis, autophagy repression, and extracellular matrix (ECM) degradation was measured by Annexin/PI staining, immunofluorescence, and immunoblot. The effect of BBR on IDD was investigated in rat. Our findings showed that BBR restored cell growth and attenuated apoptosis in IL-1 beta-induced NP cells. BBR also prevented the IL-1 beta-induced ECM degradation through regulating ECM-related enzymes and factors. Additionally, BBR significantly activated autophagy repressed by IL-1 beta. Autophagy stimulated by BBR was diminished by the inhibition of the AMPK/mTOR/Ulk1 signaling pathway. In vivo study also showed BBR attenuated intervertebral disc degeneration. BBR could attenuate NP cells apoptosis and ECM degradation induced by IL-1 beta through autophagy by the AMPK/mTOR/Ulk1 pathway. This study suggests BBR might function as an AMPK activator to alleviate IDD progression.

引用

页数：11

共 45 条

[1] What is intervertebral disc degeneration, and what causes it? [J].

Adams, Michael A. ;

Roughley, Peter J. .

SPINE, 2006, 31 (18) :2151-2161

[2] Classification of age-related changes in lumbar intervertebral discs [J].

Boos, N ;

Weissbach, S ;

Rohrbach, H ;

Weiler, C ;

Spratt, KF ;

Nerlich, AG .

SPINE, 2002, 27 (23) :2631-2644

[3] Metformin protects against apoptosis and senescence in nucleus pulposus cells and ameliorates disc degeneration in vivo [J].

Chen, Deheng ;

Xia, Dongdong ;

Pan, Zongyou ;

Xu, Daoliang ;

Zhou, Yifei ;

Wu, Yaosen ;

Cai, Ningyu ;

Tang, Qian ;

Wang, Chenggui ;

Yan, Meijun ;

Zhang, Jing Jie ;

Zhou, Kailiang ;

Wang, Quan ;

Feng, Yongzeng ;

Wang, Xiangyang ;

Xu, Huazi ;

Zhang, Xiaolei ;

Tian, Naifeng .

CELL DEATH & DISEASE, 2016, 7 :e2441-e2441

[4] Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1β/NF-κB-NLRP3 inflammasome positive feedback loop [J].

Chen, Fan ;

Jiang, Guowei ;

Liu, Hui ;

Li, Zemin ;

Pei, Yuxin ;

Wang, Hua ;

Pan, Hehai ;

Cui, Haowen ;

Long, Jun ;

Wang, Jianru ;

Zheng, Zhaomin .

BONE RESEARCH, 2020, 8 (01)

[5] Celastrol reduces IL-1β induced matrix catabolism, oxidative stress and inflammation in human nucleus pulposus cells and attenuates rat intervertebral disc degeneration in vivo [J].

Chen, Jian ;

Xuan, Jun ;

Gu, Yun-Tao ;

Shi, Ke-Si ;

Xie, Jun-Jun ;

Chen, Jiao-Xiang ;

Zheng, Zeng-Ming ;

Chen, Yu ;

Chen, Xi-Bang ;

Wu, Yao-Sen ;

Zhang, Xiao-Lei ;

Wang, Xiang-Yang .

BIOMEDICINE & PHARMACOTHERAPY, 2017, 91 :208-219

[6] Mfn2 is involved in intervertebral disc degeneration through autophagy modulation [J].

Chen, Y. ;

Lin, J. ;

Chen, J. ;

Huang, C. ;

Zhang, Z. ;

Wang, J. ;

Wang, K. ;

Wang, X. .

OSTEOARTHRITIS AND CARTILAGE, 2020, 28 (03) :363-374

[7] Berberine suppresses apoptosis and extracellular matrix (ECM) degradation in nucleus pulposus cells and ameliorates disc degeneration in a rodent model [J].

Chen, Yu ;

Zheng, Zengming ;

Wang, Jianle ;

Tang, Chengxuan ;

Khor, Sinan ;

Chen, Jian ;

Chen, Xibang ;

Zhang, Zengjie ;

Tang, Qian ;

Wang, Chenggui ;

Lou, Yiting ;

Wang, Zhouguang ;

Xiao, Jian ;

Wang, Xiangyang .

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2018, 14 (06) :682-692

[8] Cell death: a review of the major forms of apoptosis, necrosis and autophagy [J].

D'Arcy, Mark S. .

CELL BIOLOGY INTERNATIONAL, 2019, 43 (06) :582-592

[9] Autophagy as a potential therapeutic target in intervertebral disc degeneration [J].

Gong, Chao-yang ;

Zhang, Hai-hong .

LIFE SCIENCES, 2021, 273

[10] Autophagy in the Degenerating Human Intervertebral Disc In Vivo Molecular and Morphological Evidence, and Induction of Autophagy in Cultured Annulus Cells Exposed to Proinflammatory Cytokines-Implications for Disc Degeneration [J].

Gruber, Helen E. ;

Hoelscher, Gretchen L. ;

Ingram, Jane A. ;

Bethea, Synthia ;

Hanley, Edward N., Jr. .

SPINE, 2015, 40 (11) :773-782

← 1 2 3 4 5 →