CLOCK stabilizes CYCLE to initiate clock function in Drosophila

被引:12
作者
Liu, Tianxin [1 ,2 ]
Mahesh, Guruswamy [1 ,2 ]
Yu, Wangjie [1 ,2 ]
Hardin, Paul E. [1 ,2 ]
机构
[1] Texas A&M Univ, Dept Biol, College Stn, TX 77845 USA
[2] Texas A&M Univ, Ctr Biol Clocks Res, College Stn, TX 77845 USA
关键词
circadian clock; Drosophila; protein stability; CYCLE; cryptochrome; CIRCADIAN OSCILLATOR; CRYPTOCHROME; TRANSCRIPTION; LIGHT; PHOSPHORYLATION; PROTEIN; CRY; PHOTORECEPTOR; NEURONS; TISSUE;
D O I
10.1073/pnas.1707143114
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The Drosophila circadian clock keeps time via transcriptional feedback loops. These feedback loops are initiated by CLOCK-CYCLE (CLK-CYC) heterodimers, which activate transcription of genes encoding the feedback repressors PERIOD and TIMELESS. Circadian clocks normally operate in similar to 150 brain pacemaker neurons and in many peripheral tissues in the head and body, but can also be induced by expressing CLK in nonclock cells. These ectopic clocks also require cyc, yet CYC expression is restricted to canonical clock cells despite evidence that cyc mRNA is widely expressed. Here we show that CLK binds to and stabilizes CYC in cell culture and in nonclock cells in vivo. Ectopic clocks also require the blue light photoreceptor CRYPTOCHROME (CRY), which is required for both light entrainment and clock function in peripheral tissues. These experiments define the genetic architecture required to initiate circadian clock function in Drosophila, reveal mechanisms governing circadian activator stability that are conserved in perhaps all eukaryotes, and suggest that Clk, cyc, and cry expression is sufficient to drive clock expression in naive cells.
引用
收藏
页码:10972 / 10977
页数:6
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