Separation of floridoside and isofloridosides by HPLC and complete 1H and 13C NMR spectral assignments for D-isofloridoside

被引：14

作者：

Bondu, Stephanie ^{[1
]}

Kervarec, Nelly

Deslandes, Eric

Pichon, Roger

机构：

[1] Univ Bretagne Occidentale, European Univ Inst, Lab Ecophysiol Biotechnol Halophytes Algues Marin, F-29280 Plouzane, France

[2] Univ Bretagne Occidentale, Lab Resonance Magnet Nucl, UFR Sci Tech, F-29285 Brest, France

来源：

CARBOHYDRATE RESEARCH | 2007年 / 342卷 / 16期

关键词：

Porphyra umbilicalis; isofloridoside; NMR; floridoside;

D O I：

10.1016/j.carres.2007.07.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isofloridosides (1-O-alpha-D-galactopyranosylglycerol) and floridoside (2-O-alpha-D-galactopyranosylglycerol) were extracted from the red alga Porphyra umbilicalis (Linne) Kutzing (Bangiales, Rhodophyta). Their separation was achieved by HPLC (NH2 P50 column) after successive purification of the crude extract by ion-exchange chromatography and HPLC (Sugar-Pak TMI column). 1D and 2D NMR spectroscopy experiments allowed to completely assign the H-1 and C-13 spectra of D-isofloridoside. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：2470 / 2473

页数：4

共 11 条

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