Channel catfish CD8α and CD8β co-receptors: Characterization, expression and polymorphism

被引：14
作者：
Quiniou, Sylvie M. A.
Sahoo, Manoranjan [1 ]
Edholm, Eva-Stina [1 ]
Bengten, Eva [1 ]
Wilson, Melanie [1 ]
机构：
[1] Univ Mississippi, Med Ctr, Dept Microbiol, Jackson, MS 39216 USA
来源：
FISH & SHELLFISH IMMUNOLOGY | 2011年 / 30卷 / 03期
关键词：
Channel catfish; CD8; Thymus; Gene structure; Single nucleotide polymorphism; T-CELL-RECEPTOR; TCR-ALPHA-CHAIN; DICENTRARCHUS-LABRAX L; GINBUNA CRUCIAN CARP; MEDIATED CYTOTOXICITY; MOLECULAR-CLONING; LYMPHOCYTE HETEROGENEITY; RAINBOW-TROUT; TELEOST FISH; BETA-CHAIN;
D O I：
10.1016/j.fsi.2011.01.011
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
In this study we report the identification and characterization of channel catfish, Ictalurus punctatus CD8 alpha and CD8 beta genes. Both genes encode predicted proteins containing a leader, a immunoglobulin superfamily V domain, a stalk/hinge region, a transmembrane region and a positively charged cytoplasmic tail (CYT) containing the conserved teleost C-X-H motif. Catfish CD8 alpha and CD8 beta are encoded as single copy genes and as in other vertebrates exhibit a conserved head to tail synteny: the CD8 beta gene is found 14.1 kb upstream of the CD8 alpha gene. Both CD8 alpha and CD8 beta transcripts showed a low degree of polymorphism. Finally, as determined by q-PCR both CD8 alpha and CD8 beta are expressed in various catfish lymphoid tissues with the highest expression observed in thymus from 2 month old catfish-fry. In the future these results will provide the basis for evaluating the role of CD8(+) CTL. and other CD8-bearing cells in response to immunization or infection in the catfish. Published by Elsevier Ltd.
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收藏
页码：894 / 901
页数：8
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