Genome Mining and Activation of a Silent PKS/NRPS Gene Cluster Direct the Production of Totopotensamides

被引：41

作者：

Chen, Ruidong ^{[1
]}

Zhang, Qingbo ^{[1
]}

Tan, Bin ^{[1
]}

Zheng, Liujuan ^{[1
]}

Li, Huixian ^{[1
]}

Zhu, Yiguang ^{[1
]}

Zhang, Changsheng ^{[1
]}

机构：

[1] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med,RNAM Ctr Marine, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China

来源：

ORGANIC LETTERS | 2017年 / 19卷 / 20期

关键词：

PEPTIDYL NUCLEOSIDE ANTIBIOTICS; POLYKETIDE SYNTHASE; LOBOPHORIN BIOSYNTHESIS; PACIDAMYCIN GROUP; VANCOMYCIN GROUP; STREPTOMYCES SP; TETR FAMILY; IDENTIFICATION; REVEALS; (S)-3,5-DIHYDROXYPHENYLGLYCINE;

D O I：

10.1021/acs.orglett.7b02878

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A92 kb silent hybrid polyketide and nonribosomal peptide gene cluster in. marine-derived Streptomyces pactum SCSIO 02999 was activated by genetically Manipulating the regulatory genes, including the knockout of two negative regulators (totR5 and totR3) and overexpression of a positive regulator totR1, to direct the production of the known totopotensamide (TPMS)-A (1) and B (3), and a novel sulfonate-containing analogue TPM C (2). Inactivation of totG led to accumulation of TPM B (3) lacking the glycosyl Moiety, which indicated TotG as a dedicated glycosyltransferase in the biosynthesis of 1 and 2.

引用

页码：5697 / 5700

页数：4

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