Red-Emitting DPSB-Based Conjugated Polymer Nanoparticles with High Two-Photon Brightness for Cell Membrane Imaging

被引：50

作者：

Liu, Peng ^{[1
,2
]}

Li, Shuang ^{[2
]}

Jin, Yaocheng ^{[1
]}

Qian, Linghui ^{[2
]}

Gao, Nengyue ^{[2
]}

Yao, Shao Q. ^{[2
]}

Huang, Fei ^{[1
]}

Xu, Qing-Hua ^{[2
]}

Cao, Yong ^{[1
]}

机构：

[1] S China Univ Technol, State Key Lab Luminescent Mat & Devices, Inst Polymer Optoelect Mat & Devices, Guangzhou 510640, Guangdong, Peoples R China

[2] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

来源：

ACS APPLIED MATERIALS & INTERFACES | 2015年 / 7卷 / 12期

关键词：

conjugated polymers; nanoparticles; energy transfer; two-photon; fluorescence; cell imaging; AGGREGATION-INDUCED EMISSION; NEAR-INFRARED FLUORESCENCE; QUANTUM-DOT; PHOTODYNAMIC THERAPY; ENERGY-TRANSFER; CROSS-SECTIONS; ORGANIC DOTS; ABSORPTION; DESIGN; PROBES;

D O I：

10.1021/acsami.5b00223

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

New red-emitting conjugated polymers have been successfully synthesized by incorporating classical two-photon absorption (TPA) units, electron-rich units, and a small amount of electron-deficient units along the polymer backbones. Water-dispersible nanoparticles (NPs) based on these polymers were also fabricated for applications in two-photon excitation fluorescence imaging of cell membrane. Through optimization of the polymer/matrix mass ratio and the initial feed concentration of the polymer solution, a high quantum yield (QY) of 24% was achieved for the red-emitting NPs in water. TPA cross section and two-photon action cross section values of these polymers at 750 nm reached up to 1000 GM and 190 GM per repeat unit in aqueous media, 2.5 x 10(5) GM and 4.7 x 10(4) GM per NP, respectively. Furthermore, these NPs displayed excellent photostability and biocompatibility. Their applications as two-photon excitation fluorescence probes for cell membrane imaging have been demonstrated in three different cell lines with excellent imaging contrast. These results demonstrated that these polymer NPs hold great potentials as excellent two-photon excitation fluorescence probes in various biological applications.

引用

页码：6754 / 6763

页数：10

共 64 条

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