Microchip PCR

被引：129

作者：

Kricka, LJ ^{[1
]}

Wilding, P ^{[1
]}

机构：

[1] Univ Penn, Med Ctr, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2003年 / 377卷 / 05期

关键词：

PCR microchip; miniaturization; DNA;

D O I：

10.1007/s00216-003-2144-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Miniaturization of genetic tests has become an important goal. This review surveys the current progress towards the miniaturization of tests based on the polymerase chain reaction (PCR). It examines the different types of PCR microchip designs, fabrication methods, and the components of a microchip PCR device. It also discusses the problems attributable to surface chemistry of microchip components ( inhibition of PCR), and the static and dynamic surface passivation strategies developed for the solution of these difficulties.

引用

页码：820 / 825

页数：6

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