Ultrasensitive immunochromatographic strip for detection of cyproheptadine

被引：18
作者：
Guo, Mengyuan [1 ,2 ,3 ,4 ]
Sun, Li [5 ]
Liu, Liqiang [1 ,2 ,3 ,4 ]
Song, Shanshan [1 ,2 ,3 ,4 ]
Kuang, Hua [1 ,2 ,3 ,4 ]
Cui, Gang [6 ]
机构：
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Jiangnan Univ, Int Joint Res Lab Biointerface & Biodetect, Wuxi 214122, Jiangsu, Peoples R China
[3] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[4] Jiangnan Univ, Collaborat Innovationctr Food Safety & Qual Contr, Wuxi 214122, Jiangsu, Peoples R China
[5] Chinese Acad Inspect & Quarantine, Yizhuang Econ & Technol Dev Zone, 11 Ronghua South Rd, Beijing, Peoples R China
[6] Yancheng Teachers Univ, Sch Marine & Biol Engn, Yancheng, Jiangsu, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2018年 / 29卷 / 01期
关键词：
Cyproheptadine; indirect competitive enzyme-linked immunosorbent assay (IC-ELISA); immunochromatographic test strip; urine; SOLID-PHASE EXTRACTION; MONOCLONAL-ANTIBODY; CAPILLARY-ELECTROPHORESIS; HUMAN PLASMA; OPTIMIZATION; SPECTROMETRY; DESIGN;
D O I：
10.1080/09540105.2018.1490395
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Using immunological and cell fusion technology, group-specific monoclonal antibodies were produced against cyproheptadine. The 50% inhibitory concentration of the antibody for cyproheptadine was 0.37ng/mL, the linear range was 0.16-0.85ng/mL, and recoveries of antibody in spiked negative pig urine were 80-120%. An ultrasensitive immunochromatographic strip, based on the antibody, was developed for the rapid detection of cyproheptadine in pig urine. The test strip had a cut-off value of 5ng/mL.
引用
收藏
页码：941 / 952
页数：12
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