BmNPV Orf 65 (Bm65) Is Identified as an Endonuclease Directly Facilitating UV-Induced DNA Damage Repair

被引:3
作者
Tang, Qi [1 ]
Liu, Yutong [1 ]
Tang, Jingjing [1 ]
Chen, Fangying [1 ]
Qi, Xinyu [1 ]
Zhu, Feifei [1 ]
Yu, Qian [1 ]
Chen, Huiqing [1 ]
Wu, Peng [1 ]
Chen, Liang [1 ]
Guo, Zhongjian [1 ]
Hu, Zhaoyang [1 ]
Ma, Shangshang [1 ]
Chen, Keping [1 ]
Li, Guohui [1 ]
机构
[1] Jiangsu Univ, Sch Life Sci, Zhenjiang, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Bombyx mori; baculovirus; BmNPV; ultraviolet radiation; DNA damage repair; Bm65; NUCLEOTIDE EXCISION-REPAIR; CHRYSODEIXIS-CHALCITES NUCLEOPOLYHEDROVIRUS; HERPES-SIMPLEX-VIRUS; GENOME SEQUENCE; BACULOVIRUS; PHOTOLYASE; REPLICATION; PROTEINS; GENE; LOCALIZATION;
D O I
10.1128/jvi.00557-22
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Baculoviruses have been used as biopesticides for the control of Lepidoptera larvae. However, solar UV radiation reduces the activity of baculovirus. In this study, an UV endonuclease, Bm65, was found encoded in the genome of Bombyx mori nuclear polyhedrosis virus (BmNPV). Bm65 (the ortholog of AcMNPV orf79) was guided by a key nuclear localization signal to enter the nucleus and accumulated at UV-induced DNA damage sites. Subsequent results further showed that Bm65-mediated DNA damage repair was not the only UV damage repair pathway of BmNPV. BmNPV also used host DNA repair proteins to repair UV-induced DNA damage. In summary, these results revealed that Bm65 was very important in UV-induced DNA damage repair of BmNPV, and BmNPV repaired UV-damaged DNA through a variety of ways. IMPORTANCE Baculovirus biopesticides are environmentally friendly insecticides and specifically infect invertebrates. UV radiation from the sunlight greatly reduces the activity of baculovirus biopesticides. However, the molecular mechanisms of most baculoviruses to repair UV-induced DNA damage remain unclear. Nucleotide excision repair (NER) is a major DNA repair pathway that removes UV-induced DNA lesions. At present, there are few reports about the nucleotide excision repair pathway in viruses. Here, we showed for the first time that the baculovirus Bm65 endonuclease actually cleaved UV-damaged DNA. Meanwhile, we found that BmNPV used both viral-encoded enzymes and host DNA damage repair proteins to reverse UV-induced DNA damage. These results will provide a reference for the research of UV damage repair of other viruses. Baculovirus biopesticides are environmentally friendly insecticides and specifically infect invertebrates. UV radiation from the sunlight greatly reduces the activity of baculovirus biopesticides.
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页数:15
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共 45 条
  • [1] Conserved domains in DNA repair proteins and evolution of repair systems
    Aravind, L
    Walker, DR
    Koonin, EV
    [J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (05) : 1223 - 1242
  • [2] Baculovirus cyclobutane pyrimidine dimer photolyases show a close relationship with lepidopteran host homologues
    Biernat, M. A.
    Ros, V. I. D.
    Vlak, J. M.
    van Oers, M. M.
    [J]. INSECT MOLECULAR BIOLOGY, 2011, 20 (04) : 457 - 464
  • [3] A Baculovirus Photolyase with DNA Repair Activity and Circadian Clock Regulatory Function
    Biernat, Magdalena A.
    Eker, Andre P. M.
    van Oers, Monique M.
    Vlak, Just M.
    van der Horst, Gijsbertus T. J.
    Chaves, Ines
    [J]. JOURNAL OF BIOLOGICAL RHYTHMS, 2012, 27 (01) : 3 - 11
  • [4] Complete replication of plasmid DNA containing a single UV-induced lesion in human cell extracts
    Carty, MP
    Lawrence, CW
    Dixon, K
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (16) : 9637 - 9647
  • [5] The ubiquitin receptor Rad23: At the crossroads of nucleotide excision repair and proteasomal degradation
    Dantuma, Nico P.
    Heinen, Christian
    Hoogstraten, Deborah
    [J]. DNA REPAIR, 2009, 8 (04) : 449 - 460
  • [6] Friedberg E.C., 1995, DNA Repair and Mutagenesis
  • [7] Chlorella virus PBCV-1 encodes a homolog of the bacteriophage T4 UV damage repair gene denV
    Furuta, M
    Schrader, JO
    Schrader, HS
    Kokjohn, TA
    Nyaga, S
    McCullough, AK
    Lloyd, RS
    Burbank, DE
    Landstein, D
    Lane, L
    VanEtten, JL
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (04) : 1551 - 1556
  • [8] Protein kinesis - Nucleocytoplasmic transport
    Gorlich, D
    Mattaj, IW
    [J]. SCIENCE, 1996, 271 (5255) : 1513 - 1518
  • [9] Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair
    Guo, Zhigang
    Qian, Limin
    Liu, Ren
    Dai, Huifang
    Zhou, Mian
    Zheng, Li
    Shen, Binghui
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2008, 28 (13) : 4310 - 4319
  • [10] Characterization of aggregate/aggresome structures formed by polyhedrin of Bombyx mori nucleopolyhedrovirus
    Guo, Zhong-Jian
    Tao, Liu-Xing
    Dong, Xian-Yun
    Yu, Meng-Han
    Tian, Ting
    Tang, Xu-Dong
    [J]. SCIENTIFIC REPORTS, 2015, 5