Effect of 1,2-indanedione on PCR-STR typing of fingerprints deposited on thermal and carbonless paper

被引：14
作者：
Yu, Pei-Hua [1 ]
Wallace, Margaret M. [1 ]
机构：
[1] CUNY, Dept Sci, CUNY John Jay Coll Criminal Justice, New York, NY 10021 USA
来源：
FORENSIC SCIENCE INTERNATIONAL | 2007年 / 168卷 / 2-3期
关键词：
forensic science; low copy number DNA; STR; PCR typing; 1,2-indanedione; fingerprints;
D O I：
10.1016/j.forsciint.2006.06.071
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
1,2-Indanedione treated fingerprints deposited on two substrates (thermal and carbonless paper) were swabbed and tested to determine the effect of the chemical on PCR-STR DNA typing. Samples from 10 post-treatment intervals were analyzed. Two extraction methods, Chelex (R) and Qiamp, were used to determine the effect of extraction procedure on the quality of the DNA profiles. The LCN DNA samples were concentrated using Microcon (R) 100 and amplified using the Profiler Plus amplification kit. 1,2-Indanedione did not adversely affect the DNA profiles obtained from the treated fingerprints. Partial DNA profiles were obtained at all post-development time frames. Both extraction methods produced comparable profiles although more '' drop-ins '' were observed with the Qiamp method. More '' drop-outs '' occurred in the DNA profiles from samples deposited on the thermal substrate compared with carbonless substrate. (C) 2006 Elsevier Ireland Ltd. All rights reserved.
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收藏
页码：112 / 118
页数：7
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