The Nrf2-ARE Pathway Is Associated With Schisandrin B Attenuating Benzo(a)pyrene-Induced HTR Cells Damages in Vitro

被引:25
作者
Dong, Qulong [1 ]
Hou, Haiyan [1 ,2 ,3 ]
Wu, Jun [4 ,5 ]
Chen, Yaqiong [1 ,6 ]
机构
[1] Logist Univ Chinese Peoples Armed Forces, Affiliated Hosp, Dept Obstet & Gynecol, Tianjin 300162, Peoples R China
[2] Chinese Acad Med Sci, Beijing 100730, Peoples R China
[3] Peking Union Med Coll, Beijing 100730, Peoples R China
[4] Univ Calif Irvine, Program Publ Hlth, Irvine, CA 92697 USA
[5] Univ Calif Irvine, Dept Epidemiol, Irvine, CA 92697 USA
[6] Tianjin Key Lab Prevent & Control Occupat & Envir, Tianjin 300162, Peoples R China
基金
中国国家自然科学基金;
关键词
benzo(a)pyrene; Schisandrin B; Nrf2-ARE pathway; HTR; POLYCYCLIC AROMATIC-HYDROCARBONS; OXIDATIVE-STRESS; DNA-DAMAGE; UP-REGULATION; EXPOSURE; INDUCTION; CANCER; PHOSPHORYLATION; ISOTHIOCYANATES; INVOLVEMENT;
D O I
10.1002/tox.22149
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
As is ubiquitous in the environmental sources, benzo(a)pyrene (BaP) has been reported to induce reprotoxicity in previous studies. Toxicity to trophoblast cells may be one key factor, but evidences were absent. We speculated that BaP can induce cytotoxicity in human trophoblast HTR-8/SVneo (HTR) cells, and Schisandrin B (Sch B) as a potential protector can inhibit the cytotoxicity. MTS assay identified that BaP induced HTR cells death while Sch B played a cytoprotective role. And after Nrf2 interference, the ability of Sch B-induced cytoprotection was declined. Furthermore, PCR, western blot, ELISA, and SOD assays were found that Sch B significantly increased the mRNA and protein expression of Nrf2, HO1, NQO1, and SOD in the Nrf2-ARE pathway, and the extents of increase were declined after Nrf2 interference. These results demonstrated that the Nrf2-ARE pathway plays an important role in Sch B attenuating BaP-induced HTR cells damages in vitro. (C) 2015 Wiley Periodicals, Inc.
引用
收藏
页码:1439 / 1449
页数:11
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