GAK: A cyclin G associated kinase contains a tensin/auxilin-like domain

被引：111

作者：

Kanaoka, Y

Kimura, SH

Okazaki, I

Ikeda, M

Nojima, H

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,DEPT MOL GENET,SUITA,OSAKA 565,JAPAN

[2] PHARM BIOTECH KK,OSAKA 532,JAPAN

[3] MED & BIOL LABS CO LTD,NAGOYA,AICHI 460,JAPAN

来源：

FEBS LETTERS | 1997年 / 402卷 / 01期

关键词：

cyclin G; CDK5; tensin; auxilin; kinase;

D O I：

10.1016/S0014-5793(96)01484-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a cDNA encoding a novel association partner of cyclin G by West-Western blotting, The cDNA encodes a protein that harbors a Ser/Thr protein kinase-like catalytic domain at the N-terminal, Hence, we named it GAK (cyclin (G) under bar-(a) under bar ssociated (k) under bar inase). The long C-terminal extension shares homology with tensin and auxilin, and contains a leucine zipper region, Co-immunoprecipitation and Western blotting showed that GAK and cyclin G associate together in vivo, GAK also co-precipitated with CDK5, and CDK5 was found to be associated with cyclin G, We also showed by BIAcore analysis that the GAK-cyclin G interaction was direct.

引用

页码：73 / 80

页数：8

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