Mechanism of processivity clamp opening by the delta subunit wrench of the clamp loader complex of E-coli DNA polymerase III

被引：205

作者：

Jeruzalmi, D ^{[1
]}

Yurieva, O ^{[1
]}

Zhao, YX ^{[1
]}

Young, M ^{[1
]}

Stewart, J ^{[1
]}

Hingorani, M ^{[1
]}

O'Donnell, M ^{[1
]}

Kuriyan, J ^{[1
]}

机构：

[1] Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10021 USA

来源：

CELL | 2001年 / 106卷 / 04期

关键词：

D O I：

10.1016/S0092-8674(01)00462-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The dimeric ring-shaped sliding clamp of E. coli DNA polymerase III (beta subunit, homolog of eukaryotic PCNA) is loaded onto DNA by the clamp loader gamma complex (homolog of eukaryotic Replication Factor C, RFC). The delta subunit of the gamma complex binds to the beta ring and opens it. The crystal structure of a beta:delta complex shows that delta, which is structurally related to the delta' and gamma subunits of the gamma complex, is a molecular wrench that induces or traps a conformational change in beta such that one of its dimer interfaces is destabilized. Structural comparisons and molecular dynamics simulations suggest a spring-loaded mechanism in which the beta ring opens spontaneously once a dimer interface is perturbed by the delta wrench.

引用

页码：417 / 428

页数：12

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