Plasmid-mediate transfer of FLO1 into industrial Saccharomyces cerevisiae PE-2 strain creates a strain useful for repeat-batch fermentations involving flocculation-sedimentation

被引：12

作者：

Gomes, Daniel G. ^{[1
]}

Guimaraes, Pedro M. R. ^{[1
]}

Pereira, Francisco B. ^{[1
]}

Teixeira, Jose A. ^{[1
]}

Domingues, Lucilia ^{[1
]}

机构：

[1] Univ Minho, Ctr Biol Engn, IBB, P-4710057 Braga, Portugal

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 108卷

关键词：

Yeast flocculation; VHG bio-ethanol production; Repeat-batch operation; Industrial Saccharomyces cerevisiae strains; BIO-ETHANOL FERMENTATIONS; HIGH-GRAVITY FERMENTATION; YEAST FLOCCULATION; GENE; TRANSFORMATION; CELLS; CONSTRUCTION; EXPRESSION; SELECTION; ALCOHOL;

D O I：

10.1016/j.biortech.2011.12.089

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The flocculation gene FLO1 was transferred into the robust industrial strain Saccharomyces cerevisiae PE-2 by the lithium acetate method. The recombinant strain showed a fermentation performance similar to that of the parental strain. In 10 repeat-batch cultivations in VHG medium with 345 g glucose/L and cell recycling by flocculation-sedimentation, an average final ethanol concentration of 142 g/L and an ethanol productivity of 2.86 g/L/h were achieved. Due to the flocculent nature of the recombinant strain it is possible to reduce the ethanol production cost because of lower centrifugation and distillation costs. (c) 2011 Elsevier Ltd. All rights reserved.

引用

页码：162 / 168

页数：7

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