Rapid and targeted introgression of fgr gene through marker-assisted backcrossing in rice (Oryza sativa L.)

被引:5
作者
Cheng, Acga [1 ,2 ]
Ismail, Ismanizan [2 ,3 ]
Osman, Mohamad [4 ]
Hashim, Habibuddin [5 ]
Zainual, Nur Samahah Mohd [2 ,6 ]
机构
[1] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Malaysia
[2] Univ Kebangsaan Malaysia, Fac Sci, Sch Biosci & Biotechnol, Bangi 43600, Selangor Darul, Malaysia
[3] Univ Kebangsaan Malaysia, Inst Syst Biol INBIOSIS, Bangi 43600, Selangor Darul, Malaysia
[4] Univ Putra Malaysia, Fac Agr, Dept Crop Sci, Serdang 43300, Malaysia
[5] MARDI, Kepala Batas 13200, Pulau Pinang, Malaysia
[6] MARDI Headquarters, Agrobiodivers & Environm Res Ctr, Serdang 43300, Selangor Darul, Malaysia
关键词
fgr gene; fragrant; marker-assisted backcrossing; recurrent parent genome; rice; QUANTITATIVE TRAIT LOCI; KERNEL ELONGATION; FOOD SECURITY; BASMATI RICE; RESISTANCE; SELECTION; AROMA; DIVERSITY; CULTIVARS; VARIETIES;
D O I
10.1139/gen-2017-0100
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
While it is crucial for developing countries like Malaysia to achieve self-sufficiency in rice (Oryza sativa L.), it is equally critical to be able to produce high-quality rice, specifically fragrant rice, which demands are often met through importation. The present study was aimed at developing high-yielding fragrant rice, in a timely and cost-effective manner. A marker-assisted backcross (MABC) approach was optimised to introgress the fragrance gene (fgr) into two high-yielding Malaysian varieties, MR84 and MR219, within two years utilising less than 50 molecular markers. Coupled with phenotypic screening, one single foreground marker (fgr-SNP) and 48 background markers were selected and utilised, revealing recovery of at least 90% of recurrent parent genome (RPG) in merely two backcross generations. Collectively, the yield potential of the developed BC2F2 lines (BLs) was higher (P > 0.05) than the donor parent, MRQ74, and similar (P < 0.05) to both the recurrent parents, MR84 and MR219. In addition, some of the developed BLs showed good grain quality, such as having long grain. We believe that this is the first report comprising the validation and utilisation of the single functional marker system (fgr-SNP) in introgressing the fgr gene into different rice varieties.
引用
收藏
页码:1045 / 1050
页数:6
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