Divalent cation interactions with oligogalacturonides

被引：18
作者：
Cescutti, P [1 ]
Rizzo, R [1 ]
机构：
[1] Univ Trieste, Dipartimento Biochim Biofis & Chim Macromol, I-34127 Trieste, Italy
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2001年 / 49卷 / 07期
关键词：
galacturonides; conformational properties; cation interaction; electrospray mass spectrometry;
D O I：
10.1021/jf001213a
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The conformational properties of high and low molecular weight galacturonides were investigated in relation to the ability of oligomers with degree of polymerization > 10 to act as elicitors of plant defense mechanisms. Oligomers from polygalacturonate were obtained by means of enzymatic hydrolysis. Two fractions exhibiting high and low average degrees of polymerization were isolated by solvent fractionation and characterized by means of electrospray mass spectrometry. The conformational behaviors of the two fractions were investigated in the presence of different divalent cations using circular dichroism. Calcium, copper, and zinc ions were able to induce a conformational transition in both fractions. When in the presence of the high molecular weight fraction, copper and zinc ions were much more effective than calcium ions, whereas the efficiency was much reduced with low molecular weight oligomers.
引用
收藏
页码：3262 / 3267
页数：6
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