Development and Characterization of EST-SSR Markers for Tricyrtis sect. Tricyrtis (Liliaceae)

被引：0

作者：

Nakamasu, Riku ^{[1
]}

Sakaguchi, Shota ^{[1
]}

Setoguchi, Hiroaki ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Human & Environm Studies, Sakyo Ku, Yoshida Nihonmatsu Cho, Kyoto 6068501, Japan

来源：

ACTA PHYTOTAXONOMICA ET GEOBOTANICA | 2020年 / 71卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

EST-SSR; genetic diversity; microsatellite loci; next generation sequencing; population genetics; Tricyrtis;

D O I：

10.18942/apg.201907

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A set of 8 polymorphic EST-SSR markers has been developed to evaluate the genetic diversity and genetic structure of the populations of Tricyrtis sect. Tricyrtis (Liliaceae) in Kyushu, which reproduces sexually and asexually by bulbils. The total number of alleles for each locus ranged from 2 to 11 with an average of 5.5, while the values of observed and expected heterozygosity ranged from 0.043 to 0.875 and 0.043 to 0.849, respectively. The combined probabilities of identity (PI = 6.0E- 07 and PI-Sib = 2.7E-03) of these markers suggest that they are useful for not only evaluating genetic diversity but also for estimating clonal diversity within populations. Cross-species amplification was evaluated for three phylogenetically related species, T. macropoda, T. setouchiensis, and T. affinis, to show that all the EST-SSR markers are transferrable among these species.

引用

页码：73 / 76

页数：4

共 8 条

[1] Doyle JJ, 1987, PHYTOCHEMISTRY B, V19, P11, DOI DOI 10.1016/0031-9422(80)85004-7
[2] MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design
Faircloth, Brant C.
[J]. MOLECULAR ECOLOGY RESOURCES, 2008, 8 (01) : 92 - 94
[3] FSTAT (Version 1.2): A computer program to calculate F-statistics
Goudet, J
[J]. JOURNAL OF HEREDITY, 1995, 86 (06) : 485 - 486
[4] NEW MICROSATELLITE MARKERS FOR TRICYRTIS MACRANTHA (CONVALLARIACEAE) AND CROSS-AMPLIFICATION IN CLOSELY RELATED SPECIES
Ohki, Noriko
Setoguchi, Hiroaki
[J]. APPLICATIONS IN PLANT SCIENCES, 2013, 1 (05):
[5] GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research
Peakall, R
Smouse, PE
[J]. MOLECULAR ECOLOGY NOTES, 2006, 6 (01): : 288 - 295
[6] PHYLOGENETIC-RELATIONSHIPS IN CROSSOSTYLIS (RHIZOPHORACEAE) INFERRED FROM RESTRICTION SITE VARIATION OF CHLOROPLAST DNA
SETOGUCHI, H
OHBA, H
[J]. JOURNAL OF PLANT RESEARCH, 1995, 108 (1089) : 87 - 92
[7] TAKAHASHI H, 1987, Acta Phytotaxonomica et Geobotanica, V38, P123
[8] Takahashi H., 1980, SCI REP FAC ED GIFU, V6, P583

← 1 →