NEW MODIFIED ELECTRODES WITH HRP IMMOBILIZED IN POLYMERIC FILMS FOR PARACETAMOL ANALYSIS

In order to establish the configurations of biosensors that are able to detect paracetamol in various matrices, it was studied the behaviour of some modified electrodes with an electroconductive polymeric film. Polypyrrole films deposition on glassy carbon and screen-printed electrodes was developped by several methods. The electroanalytical characterization of the deposited films was performed by chronoamperometry in the presence of an enzyme immobilized into the polymeric films. Chronoamperometric study performed with glassy carbon and planar electrodes based on graphite, modified with horseradish peroxidase embedded in polypyrrole films, proved the existence of interactions between the immobilized enzyme and the electroactive species of paracetamol (N-acetyl-p-benzo-quinone imine-NAPQI), enzymatically generated in the presence of hydrogen peroxide.