Differential Wound Healing Capacity of Mesenchymal Stem Cell-Derived Exosomes Originated From Bone Marrow, Adipose Tissue and Umbilical Cord Under Serum- and Xeno-Free Condition

被引：117

作者：

Diem Huong Hoang ^{[1
,3
]}

Tu Dac Nguyen ^{[1
,2
]}

Hoang-Phuong Nguyen ^{[1
,3
]}

Xuan-Hung Nguyen ^{[1
,3
]}

Phuong Thi Xuan Do ^{[1
,4
]}

Van Duc Dang ^{[1
,4
]}

Phuong Thi Minh Dam ^{[1
,3
]}

Hue Thi Hong Bui ^{[1
,3
]}

Mai Quynh Trinh ^{[1
,3
]}

Duc Minh Vu ^{[1
,3
]}

Nhung Thi My Hoang ^{[1
,4
]}

Liem Nguyen Thanh ^{[1
,3
]}

Uyen Thi Trang Than ^{[1
,3
]}

机构：

[1] Vinmec Hlth Care Syst, Vinmec Res Inst Stem Cell & Gene Technol VRISG, Hanoi, Vietnam

[2] Vinmec Healthcare Syst, Vinmec Hightech Ctr, Hanoi, Vietnam

[3] Vin Univ, Coll Hlth Sci, Hanoi, Vietnam

[4] Viet Nam Univ, Univ Sci, Hanoi, Vietnam

来源：

FRONTIERS IN MOLECULAR BIOSCIENCES | 2020年 / 7卷

关键词：

exosomes; mesenchymal stem cells; BMMSC-derived exosomes; ADMSC-derived exosomes; UCMSC-derived exosomes; growth factors; wound healing; ENHANCE ANGIOGENESIS; DERMAL FIBROBLASTS; PROGENITOR CELLS; PROLIFERATION; MIGRATION; GROWTH; EXPRESSION;

D O I：

10.3389/fmolb.2020.00119

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exosomes are nano-scale and closed membrane vesicles which are promising for therapeutic applications due to exosome-enclosed therapeutic molecules such as DNA, small RNAs, proteins and lipids. Recently, it has been demonstrated that mesenchymal stem cell (MSC)-derived exosomes have capacity to regulate many biological events associated with wound healing process, such as cell proliferation, cell migration and blood vessel formation. This study investigated the regenerative potentials for cutaneous tissue, in regard to growth factors associated with wound healing and skin cell proliferation and migration, by exosomes released from primary MSCs originated from bone marrow (BM), adipose tissue (AD), and umbilical cord (UC) under serum- and xeno-free condition. We found crucial wound healing-mediated growth factors, such as vascular endothelial growth factor A (VEGF-A), fibroblast growth factor 2 (FGF-2), hepatocyte growth factor (HGF), and platelet-derived growth factor BB (PDGF-BB) in exosomes derived from all three MSC sources. However, expression levels of these growth factors in exosomes were influenced by MSC origins, especially transforming growth factor beta (TGF-beta) was only detected in UCMSC-derived exosomes. All exosomes released by three MSCs sources induced keratinocyte and fibroblast proliferation and migration; and, the induction of cell migration is a dependent manner with the higher dose of exosomes was used (20 mu g), the faster migration rate was observed. Additionally, the influences of exosomes on cell proliferation and migration was associated with exosome origins and also target cells of exosomes that the greatest induction of primary dermal fibroblasts belongs to BMMSC-derived exosomes and keratinocytes belongs to UCMSC-derived exosomes. Data from this study indicated that BMMSCs and UCMSCs under clinical condition secreted exosomes are promising to develop into therapeutic products for wound healing treatment.

引用

页数：13

共 61 条

[1] Vascular endothelial growth factor mediates the therapeutic efficacy of mesenchymal stem cell-derived extracellular vesicles against neonatal hyperoxic lung injury [J].