Hesperetin alleviated glucocorticoid-induced inhibition of osteogenic differentiation of BMSCs through regulating the ERK signaling pathway

被引：13
作者：
Liu, Ling [1 ,2 ]
Zheng, Jie [1 ,2 ]
Yang, YaZhen [1 ,2 ]
Ni, Lingjuan [1 ,2 ]
Chen, Hongyu [1 ,2 ]
Yu, Dongrong [1 ,2 ]
机构：
[1] Hangzhou Hosp Tradit Chinese Med, Dept Nephrol, Tiyuchang Rd 453, Hangzhou 310007, Zhejiang, Peoples R China
[2] Hangzhou Hosp Tradit Chinese Med, Dept Nephrol, Management Kidney Dis, Key Lab Zhejiang Prov, Tiyuchang Rd 453, Hangzhou 310007, Peoples R China
来源：
MEDICAL MOLECULAR MORPHOLOGY | 2021年 / 54卷 / 01期
关键词：
Glucocorticoid-induced osteoporosis; Hesperetin; Bone marrow mesenchymal stem cells; ERK; MESENCHYMAL STEM-CELLS; INDUCED OSTEOPOROSIS; PREVENTION; MANAGEMENT;
D O I：
10.1007/s00795-020-00251-9
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The objective of this study is to investigate the protective role of hesperetin for the glucocorticoid-induced osteoporosis (GIOP) and related mechanisms. In this study, we investigated the protective effects of hesperetin on dexamethasone (DEX)-induced osteogenic inhibition in bone marrow mesenchymal stem cells (BMSCs). The mineralization, real-time quantitative polymerase chain reaction assays (RT-qPCR), immunofluorescence and western blot were used to assess the protective effects of hesperetin in DEX-treated BMSCs during osteogenic differentiation. Our results showed that hesperetin promoted alkaline phosphatase (ALP) activity and the mineralization in DEX-treated BMSCs during osteogenic differentiation. The expression of osteogenic mRNA and proteins further confirmed the protective effect of hesperetin in DEX-treated BMSCs. Furthermore, hesperetin activated ERK signal pathway in DEX-treated BMSCs. ERK inhibitor U0126 could abolish the protective effect of hesperein in DEX-treated BMSCs. In conclusion, our study demonstrated that hesperetin alleviated glucocorticoid-induced inhibition of osteogenic differentiation through ERK signal pathway in BMSCs. It may be a potential therapeutic agent for protecting against glucocorticoid-induced osteoporosis.
引用
收藏
页码：1 / 7
页数：7
相关论文
共 50 条
  • [41] LL-37 inhibits LPS-induced inflammation and stimulates the osteogenic differentiation of BMSCs via P2X7 receptor and MAPK signaling pathway
    Yu, Xiaoran
    Quan, Jingjing
    Long, Weilin
    Chen, Huimin
    Wang, Ruoxun
    Guo, Jiaxin
    Lin, Xiaoxuan
    Mai, Sui
    EXPERIMENTAL CELL RESEARCH, 2018, 372 (02) : 178 - 187
  • [42] miR-122-5p targets GREM2 to protect against glucocorticoid-induced endothelial damage through the BMP signaling pathway
    Huang, Xianzhe
    Jie, Shuo
    Li, Wenzhao
    Li, Hui
    Ni, Jiangdong
    Liu, Chan
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2022, 544
  • [43] lncRNA HHIP-AS1/HHIP modulates osteogenic differentiation of BM-MSCs by regulating Hedgehog signaling pathway
    Yin, Xin-Hu
    Wang, Xiao-Yuan
    Liu, Shi-Chang
    Yan, Liang
    He, Bao-Rong
    Hao, Ding-Jun
    Yang, Ming
    Liu, Zhong-Kai
    AGING-US, 2022, 14 (21): : 8839 - 8855
  • [44] Bushen huoxue decoction inhibits RANKL-stimulated osteoclastogenesis and glucocorticoid-induced bone loss by modulating the NF-κB, ERK, and JNK signaling pathways
    Liu, Yamei
    Fu, Binlan
    Li, Xiaoman
    Chen, Chen
    Li, Xican
    Xu, Liangliang
    Wang, Bin
    FRONTIERS IN PHARMACOLOGY, 2022, 13
  • [45] MagT1 regulated the odontogenic differentiation of BMMSCs induced byTGC-CM via ERK signaling pathway
    Zheng, Jian-mao
    Kong, Yuan-yuan
    Li, Yao-yin
    Zhang, Wen
    STEM CELL RESEARCH & THERAPY, 2019, 10 (1)
  • [46] Inhibition of RANKL-induced osteoclastogenesis through the suppression of the ERK signaling pathway by astragaloside IV and attenuation of titanium-particle-induced osteolysis
    Li, Mingjun
    Wang, Wengang
    Geng, Li
    Qin, Yanru
    Dong, Wenjie
    Zhang, Xudong
    Qin, An
    Zhang, Mingzhi
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2015, 36 (05) : 1335 - 1344
  • [47] Osteoactivin inhibits dexamethasone-induced osteoporosis through up-regulating integrin β1 and activate ERK pathway
    Hu, He
    Li, Zhehai
    Lu, Min
    Yun, Xinyi
    Li, Wei
    Liu, Caiyun
    Guo, Ai
    BIOMEDICINE & PHARMACOTHERAPY, 2018, 105 : 66 - 72
  • [48] Inhibition of heat shock protein 90 rescues glucocorticoid-induced bone loss through enhancing bone formation
    Chen, Haixiao
    Xing, Ji
    Hu, Xinhua
    Chen, Lihua
    Lv, Haiyan
    Xu, Chengyun
    Hong, Dun
    Wu, Ximei
    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2017, 171 : 236 - 246
  • [49] Regulation of Wnt Signaling Pathway by Costic Acid Derivative, An Efficient Strategy for Treatment of Glucocorticoid-Induced Osteoporosis in Rat Model
    Jiang, Xue
    Kong, Xiangying
    CHEMISTRYSELECT, 2023, 8 (13):
  • [50] Kruppel-like factor 4 promotes the proliferation and osteogenic differentiation of BMSCs through SOX2/IGF2 pathway
    Fei, Zhengguo
    Sun, Chunguang
    Peng, Xiaoqiang
    Han, Mu
    Zhou, Qijia
    ACTA BIOCHIMICA POLONICA, 2022, 69 (02) : 349 - 355
12345