The Paf1 complex promotes displacement of histones upon rapid induction of transcription by RNA polymerase II

被引：21
|
作者：
Marton, Heather A.
Desiderio, Stephen
机构：
[1] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, Program Immunol, Inst Cell Engn, Baltimore, MD 21205 USA
来源：
BMC MOLECULAR BIOLOGY | 2008年 / 9卷
关键词：
D O I：
10.1186/1471-2199-9-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Background: The yeast Paf1 protein complex is required for efficient transcription elongation by RNA polymerase II (RNA pol II), but the precise role of the complex has been unclear. Results: Here we show that depletion of the Ctr9 or Paf1 component of the Paf1 complex delays the loss of histones from the GALI gene upon induction. This delay in histone removal is accompanied by a decrease in association of RNA pol II with GALI and altered distribution of the polymerase along the locus. Conclusion: These observations may explain why initial induction of GAL transcripts is reduced in Ctr9- or Paf1-deficient cells, and is consistent with a model suggesting that the Paf1 complex and the histone modifications that it mediates increase efficiency of transcriptional elongation by promoting nucleosomal destabilization and histone removal.
引用
收藏
页数：13
相关论文
共 50 条
  • [1] The Paf1 complex: Platform or player in RNA polymerase II transcription?
    Jaehning, Judith A.
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2010, 1799 (5-6): : 379 - 388
  • [2] The Paf1 complex is required for efficient transcription elongation by RNA polymerase I
    Zhang, Yinfeng
    Sikes, Martha L.
    Beyer, Ann L.
    Schneider, David A.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (07) : 2153 - 2158
  • [3] Direct interactions between the Paf1 complex and a cleavage and polyadenylation factor are revealed by dissociation of Paf1 from RNA polymerase II
    Nordick, Kristen
    Hoffman, Matthew G.
    Betz, Joan L.
    Jaehning, Judith A.
    EUKARYOTIC CELL, 2008, 7 (07) : 1158 - 1167
  • [4] The Paf1 complex has functions independent of actively transcribing RNA polymerase II
    Mueller, CL
    Porter, SE
    Hoffman, MG
    Jaehning, JA
    MOLECULAR CELL, 2004, 14 (04) : 447 - 456
  • [5] The Paf1 complex is required for RNA polymerase II removal in response to DNA damage
    Chen, Feilong
    Liu, Beibei
    Zhou, Hao
    Long, Jiafu
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2022, 119 (40)
  • [6] Coordinated regulation of RNA polymerase II pausing and elongation progression by PAF1
    Wang, Zhenning
    Song, Aixia
    Xu, Hao
    Hu, Shibin
    Tao, Bolin
    Peng, Linna
    Wang, Jingwen
    Li, Jiabei
    Yu, Jiali
    Wang, Li
    Li, Ze
    Chen, Xizi
    Wang, Mengyun
    Chi, Yayun
    Wu, Jiong
    Xu, Yanhui
    Zheng, Hai
    Chen, Fei Xavier
    SCIENCE ADVANCES, 2022, 8 (13)
  • [7] PAF1, a Molecular Regulator of Promoter-Proximal Pausing by RNA Polymerase II
    Chen, Fei Xavier
    Woodfin, Ashley R.
    Gardini, Alessandro
    Rickels, Ryan A.
    Marshall, Stacy A.
    Smith, Edwin R.
    Shiekhattar, Ramin
    Shilatifard, Ali
    CELL, 2015, 162 (05) : 1003 - 1015
  • [8] Transcription recycling assays identify PAF1 as a driver for RNA Pol II recycling
    Zhong Chen
    William Hankey
    Yue Zhao
    Jeff Groth
    Furong Huang
    Hongyan Wang
    Alexandre Rosa Campos
    Jiaoti Huang
    Robert G. Roeder
    Qianben Wang
    Nature Communications, 12
  • [9] Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex
    Mueller, CL
    Jaehning, JA
    MOLECULAR AND CELLULAR BIOLOGY, 2002, 22 (07) : 1971 - 1980
  • [10] Separation of the Saccharomyces cerevisiae Paf1 complex from RNA polymerase II results in changes in its subnuclear localization
    Porter, SE
    Penheiter, KL
    Jaehning, JA
    EUKARYOTIC CELL, 2005, 4 (01) : 209 - 220
12345