Quality assessment of microarray experiments

被引：8
|
作者：
Carter, DE
Robinson, JF
Allister, EM
Huff, MW
Hegele, RA
机构：
[1] John P Robarts Res Inst, London Reg Genom Ctr, London, ON N6A 5K8, Canada
[2] John P Robarts Res Inst, Blackburn Cardiovasc Genet Lab, London, ON N6A 5K8, Canada
[3] John P Robarts Res Inst, Vasc Biol Grp, London, ON N6A 5K8, Canada
来源：
CLINICAL BIOCHEMISTRY | 2005年 / 38卷 / 07期
关键词：
RNA; oligonucleotide microarrays; data quality; gene expression; microarray analysis;
D O I：
10.1016/j.clinbiochem.2005.04.010
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Objectives: We describe a quality assurance procedure to maximize the value of oligonucleotide microarray expression profiles. Design, methods, and results: Background microarray noise was 82.2 +/- 54.5 and 51.8 +/- 12.4 units, respectively, before and after enacting the program (P < 0.0001). We also noted improved concordance of microarray expression fold-changes for selected genes with results of RT-PCR validation. Conclusions: This multi-step procedure, including quantification of RNA sample degradation and detection of outlier data points, has increased data quality from our microarray facility. (C) 2005 The Canadian Society of Clinical Chemists. All rights reserved.
引用
收藏
页码：639 / 642
页数：4
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